Rabbit anti-Human ARHGAP17 Polyclonal Antibody | anti-ARHGAP17 antibody

ARHGAP17, NT (ARHGAP17, RICH1, Rho GTPase-activating protein 17, Rho-type GTPase-activating protein 17, RhoGAP interacting with CIP4 homologs protein 1) (FITC)

Cat. Num. AAA6274855

• Gene Names: ARHGAP17; PP367; RICH1; WBP15; MST066; MST110; NADRIN; PP4534; RICH1B; MSTP038; MSTP066; MSTP110
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARHGAP17; Polyclonal Antibody; NT (ARHGAP17; RICH1; Rho GTPase-activating protein 17; Rho-type GTPase-activating protein 17; RhoGAP interacting with CIP4 homologs protein 1) (FITC); RhoGAP interacting with CIP4 homologs protein 1; anti-ARHGAP17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ARHGAP17 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ARHGAP17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-74 amino acids from the N-terminal region of human ARHGAP17.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARHGAP17 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ARHGAP17 antibody detected the ARHGAP17 protein (arrow).)

Flow Cytometry (FC/FACS)

(ARHGAP17 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ARHGAP17 antibody

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA (MIM 165390), RAC1 (MIM 602048), and CDC42 (MIM 116952).[supplied by OMIM].

Product Categories/Family for anti-ARHGAP17 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Olsen, J.V., et al. Cell 127(3):635-648(2006). Wells, C.D., et al. Cell 125(3):535-548(2006). Richnau, N., et al. J. Biol. Chem. 276(37):35060-35070(2001). Reczek, D., et al. J. Cell Biol. 153(1):191-206(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 54860079
• NCBI GeneID: 55114
• NCBI Accession #: NP_001006635.1
• NCBI GenBank Nucleotide #: NM_001006634.1
• UniProt Accession #: Q68EM7; Q6ZUS4; Q7Z2F2; Q8NDG2; Q96KS2; Q96KS3; Q96SS8; Q9BVF6; Q9H8U5; Q9NW54; A8K6M6
• Molecular Weight: 95,437 Da
• NCBI Official Full Name: rho GTPase-activating protein 17 isoform 1
• NCBI Official Synonym Full Names: Rho GTPase activating protein 17
• NCBI Official Symbol: ARHGAP17
• NCBI Official Synonym Symbols: PP367; RICH1; WBP15; MST066; MST110; NADRIN; PP4534; RICH1B; MSTP038; MSTP066; MSTP110
• NCBI Protein Information: rho GTPase-activating protein 17; RICH-1; rho-type GTPase-activating protein 17; RhoGAP interacting with CIP4 homologs 1; rhoGAP interacting with CIP4 homologs protein 1; neuron-associated developmentally regulated protein
• UniProt Protein Name: Rho GTPase-activating protein 17
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: ARHGAP17
• UniProt Synonym Gene Names: RICH1; RICH-1
• UniProt Entry Name: RHG17_HUMAN

NCBI Description

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA (MIM 165390), RAC1 (MIM 602048), and CDC42 (MIM 116952).[supplied by OMIM, Apr 2004]

Uniprot Description

ARHGAP17: Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activitor in vitro for RAC1. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: tight junction; plasma membrane; cytosol

Molecular Function: protein binding; SH3 domain binding; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity