Rabbit anti-Human ARFGEF1 Polyclonal Antibody | anti-ARFGEF1 antibody
ARFGEF1 Antibody
Gene Names
ARFGEF1; BIG1; P200; ARFGEP1; DKFZP434L057; D730028O18Rik
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
ARFGEF1; Polyclonal Antibody; ARFGEF1 Antibody; ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited); ADP-ribosylation factor guanine nucleotide exchange factor 1; ARF GEP1; ARFGEP1; Brefeldin A inhibited GEP 1; Brefeldin A inhibited guanine nucleotide exchange protein 1; P200; p200 ARF guanine nucleotide exchange factor; anti-ARFGEF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
ARFGEF1 Antibody detects endogenous levels of ARFGEF1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ARFGEF1 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human ARFGEF1.
Conjugation
Unconjugated
Post Translational Modifications
Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.
Subunit Structure
Homodimer. Interacts with ARFGEF2/BIG2; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2. Interacts with MYO9B. Interacts with PRKAR1A and PRKAR2A. Interacts with PPP1CC. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA. Interacts with DPY30. Interacts with PDE3A. Interacts with KANK1. Interacts with TBC1D22A and TBC1D22B. Interacts (via N-terminus) with ARL1.
Tissue Specificity
Expressed in placenta, lung, heart, brain, kidney and pancreas.
Function
Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.
Note
Translocates from cytoplasm to membranes and nucleus upon cAMP treatment.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-ARFGEF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
208,767 Da
NCBI Official Full Name
brefeldin A-inhibited guanine nucleotide-exchange protein 1
NCBI Official Synonym Full Names
ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
NCBI Official Symbol
ARFGEF1
NCBI Official Synonym Symbols
BIG1; P200; ARFGEP1; DKFZP434L057; D730028O18Rik
NCBI Protein Information
brefeldin A-inhibited guanine nucleotide-exchange protein 1; p200 ARF-GEP1; OTTHUMP00000227052; OTTHUMP00000227095; brefeldin A-inhibited GEP 1; p200 ARF guanine nucleotide exchange factor
UniProt Protein Name
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
UniProt Gene Name
ARFGEF1
UniProt Synonym Gene Names
ARFGEP1; BIG1
UniProt Entry Name
BIG1_HUMAN
Similar Products
Product Notes
The ARFGEF1 arfgef1 (Catalog #AAA9629938) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARFGEF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARFGEF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the ARFGEF1 arfgef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARFGEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
