Western Blot (WB)
(ARFGAP2 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARFGAP2 antibody detected the ARFGAP2 protein (arrow).)
Rabbit ARFGAP2 Polyclonal Antibody | anti-ARFGAP2 antibody
ARFGAP2 Antibody (Center)
Gene Names
ARFGAP2; IRZ; ZFP289; ZNF289; NBLA10535
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARFGAP2; Polyclonal Antibody; ARFGAP2 Antibody (Center); ADP-ribosylation factor GTPase-activating protein 2; ARF GAP 2; GTPase-activating protein ZNF289; Zinc finger protein 289; ZNF289; anti-ARFGAP2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARFGAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the Central region of human ARFGAP2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.47 (varies by lot)
Sequence Positions
276-305
Sequence Length
252
Applicable Applications for anti-ARFGAP2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(ARFGAP2 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARFGAP2 antibody detected the ARFGAP2 protein (arrow).)
Western Blot (WB)
(ARFGAP2 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARFGAP2 antibody detected the ARFGAP2 protein (arrow).)
Related Product Information for anti-ARFGAP2 antibody
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
References
Saitoh, A., et al. J. Biol. Chem. 284(20):13948-13957(2009)
Weimer, C., et al. J. Cell Biol. 183(4):725-735(2008)
Frigerio, G., et al. Traffic 8(11):1644-1655(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Schmid, E.M., et al. PLoS Biol. 4 (9), E262 (2006) :
Weimer, C., et al. J. Cell Biol. 183(4):725-735(2008)
Frigerio, G., et al. Traffic 8(11):1644-1655(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Schmid, E.M., et al. PLoS Biol. 4 (9), E262 (2006) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56720
NCBI Official Full Name
ADP-ribosylation factor GTPase-activating protein 2 isoform 2
NCBI Official Synonym Full Names
ADP-ribosylation factor GTPase activating protein 2
NCBI Official Symbol
ARFGAP2
NCBI Official Synonym Symbols
IRZ; ZFP289; ZNF289; NBLA10535
NCBI Protein Information
ADP-ribosylation factor GTPase-activating protein 2
UniProt Protein Name
ADP-ribosylation factor GTPase-activating protein 2
Protein Family
UniProt Gene Name
ARFGAP2
UniProt Synonym Gene Names
ZNF289; ARF GAP 2
UniProt Entry Name
ARFG2_HUMAN
Uniprot Description
ZNF289: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Protein type: GAPs, ARF; GAPs
Chromosomal Location of Human Ortholog: 11p11.2-p11.12
Cellular Component: Golgi membrane; Golgi apparatus; cytoplasm; plasma membrane; nucleolus
Molecular Function: zinc ion binding
Biological Process: vesicle-mediated transport; protein transport; positive regulation of GTPase activity
Research Articles on ARFGAP2
1. analysis of the distinct role of subcomplexes of the COPI coat in the regulation of ArfGAP2 activity
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Product Notes
The ARFGAP2 arfgap2 (Catalog #AAA9203224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 276-305. The ARFGAP2 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ARFGAP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ARFGAP2 arfgap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARFGAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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