Rabbit anti-Human ARF3 Polyclonal Antibody | anti-ARF3 antibody

ARF3, ID (D93) (ADP-ribosylation Factor 3) (MaxLight 750)

Cat. Num. AAA6461728

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ARF3; Polyclonal Antibody; ID (D93) (ADP-ribosylation Factor 3) (MaxLight 750); anti-ARF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARF3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-ARF3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ARF3 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ARF3 antibody (Center D93) in MCF-7 cell line lysates (35ug/lane). ARF3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ARF3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Li, F., FEBS Lett. 524(1-3), 49-53 (2002). 2. Lee, F.J., J. Biol. Chem. 267(34), 24441-24445 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4502203
• NCBI GeneID: 377
• NCBI Accession #: NP_001650
• NCBI GenBank Nucleotide #: NM_001659.2
• UniProt Accession #: P61204; P16587; A8K6G8; B7ZB63
• Molecular Weight: 22.8 kDa (201aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ADP-ribosylation factor 3
• NCBI Official Synonym Full Names: ADP ribosylation factor 3
• NCBI Official Symbol: ARF3
• NCBI Protein Information: ADP-ribosylation factor 3
• UniProt Protein Name: ADP-ribosylation factor 3
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF3
• UniProt Entry Name: ARF3_HUMAN

NCBI Description

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF3: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi membrane; perinuclear region of cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: vesicle-mediated transport; protein transport; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process