Rabbit anti-Human ARC Polyclonal Antibody | anti-NOL3 antibody

ARC Antibody

Cat. Num. AAA151498

• Gene Names: NOL3; ARC; FCM; MYP; NOP; NOP30
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: ARC Antibody is affinity chromatography purified via peptide column.
• Synonyms: ARC; Polyclonal Antibody; ARC Antibody; FCM; MYP; NOP; NOP30; Nucleolar protein 3; Apoptosis repressor with CARD; Myp; nucleolar protein 3 (apoptosis repressor with CARD domain); anti-NOL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ARC Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 208

• Applicable Applications for anti-NOL3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ARC antibody can be used for detection of ARC by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ARC antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ARC.
• Buffer: ARC Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ARC antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ARC in (H) HeLa, (K) K562, and (A) A549 whole cell lysates with ARC antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ARC in human heart tissue with ARC antibody at 5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ARC in human skeletal muscle with ARC antibody at 5 μg/mL.)

Related Product Information for anti-NOL3 antibody

ARC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 4505419
• NCBI GeneID: 8996
• NCBI Accession #: NP_003937
• NCBI GenBank Nucleotide #: NM_003946.6
• UniProt Accession #: O60936; O60937; B4DFL0
• Molecular Weight: Predicted: 23 kDa; ; Observed: 25 kDa
• NCBI Official Full Name: nucleolar protein 3 isoform MYP
• NCBI Official Synonym Full Names: nucleolar protein 3 (apoptosis repressor with CARD domain)
• NCBI Official Symbol: NOL3
• NCBI Official Synonym Symbols: ARC; FCM; MYP; NOP; NOP30
• NCBI Protein Information: nucleolar protein 3; nucleolar protein of 30 kDa; muscle-enriched cytoplasmic protein
• UniProt Protein Name: Nucleolar protein 3
• Protein Family: Arcelin
• UniProt Gene Name: NOL3
• UniProt Synonym Gene Names: ARC; NOP; Myp; Nop30
• UniProt Entry Name: NOL3_HUMAN

NCBI Description

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

Isoform 1: May be involved in RNA splicing.