Goat ARAP3 Polyclonal Antibody | anti-CENTD3 antibody

Goat anti-ARAP3 Antibody

Cat. Num. AAA421192

• Gene Names: ARAP3; DRAG1; CENTD3
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ARAP3; Polyclonal Antibody; Goat anti-ARAP3 Antibody; CENTD3; centaurin; delta 3; FLJ21065; ARF-GAP; RHO-GAP; ankyrin repeat and plekstrin homology domains-containing protein 3; PtdIns(3; 4; 5)P3-binding protein; phosphoinositide binding protein; ArfGAP with RhoGAP domain; ankyrin repeat and PH domain 3; CENTD3 antibody; delta 3 antibody; ARAP3 antibody; FLJ21065 antibody; ankyrin repeat and plekstrin homology domains-containing protein 3 antibody; 5)P3-binding protein antibody; phosphoinositide binding protein antibody; ankyrin repeat and PH domain 3 antibody; DRAG1 antibody; Arf and Rho GAP adapter protein 3 antibody; anti-CENTD3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CTSSPPSSQPLT
• Sequence Length: 1544

• Applicable Applications for anti-CENTD3 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: This antibody has been successfully used in WB on scirrhous human gastric cancer cell lines.; Immunoprecipitation: This antibody has been successfully used in IP on scirrhous human gastric cancer cell lines.
• Immunogen: Peptide with sequence CTSSPPSSQPLT, from the C Terminus of the protein sequence according to NP_071926.4.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 21264337
• NCBI GeneID: 64411
• NCBI Accession #: NP_071926.4
• NCBI GenBank Nucleotide #: NM_022481.5
• Molecular Weight: 131,986 Da
• NCBI Official Full Name: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
• NCBI Official Synonym Full Names: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
• NCBI Official Symbol: ARAP3
• NCBI Official Synonym Symbols: DRAG1; CENTD3
• NCBI Protein Information: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
• UniProt Protein Name: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
• UniProt Gene Name: ARAP3
• UniProt Synonym Gene Names: CENTD3; Cnt-d3
• UniProt Entry Name: ARAP3_HUMAN

NCBI Description

This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Sep 2015]

Uniprot Description

ARAP3: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts on ARF6, RAC1, RHOA and CDC42. Plays a role in the internalization of anthrax toxin.

Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, ARF

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; lamellipodium; plasma membrane; ruffle

Molecular Function: GTPase activator activity; metal ion binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding

Biological Process: cytoskeleton organization and biogenesis; negative regulation of cell migration; negative regulation of Rac protein signal transduction; negative regulation of Rho protein signal transduction; positive regulation of GTPase activity; regulation of cell shape; regulation of small GTPase mediated signal transduction; signal transduction; vesicle-mediated transport