Rabbit anti-Human ARAP2 Polyclonal Antibody | anti-ARAP2 antibody

ARAP2, NT (ARAP2, CENTD1, KIAA0580, Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2, Centaurin-delta-1, Protein PARX) (Biotin)

Cat. Num. AAA6274689

• Gene Names: ARAP2; PARX; CENTD1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARAP2; Polyclonal Antibody; NT (ARAP2; CENTD1; KIAA0580; Arf-GAP with Rho-GAP domain; ANK repeat and PH domain-containing protein 2; Centaurin-delta-1; Protein PARX) (Biotin); Protein PARX; anti-ARAP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ARAP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-93 amino acids from the N-terminal region of human ARAP2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARAP2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ARAP2 antibody detected the ARAP2 protein (arrow).)

Related Product Information for anti-ARAP2 antibody

The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics.

Product Categories/Family for anti-ARAP2 antibody

Antibodies; KIAA; orf

References

Gavicherla, B., et al. Infect. Immun. 78(11):4532-4541(2010). Liu, C., et al. Mol. Med. 14 (9-10), 575-581 (2008): Yoon, H.Y., et al. J. Cell. Sci. 119 (PT 22), 4650-4666 (2006): Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 21264592
• NCBI GeneID: 116984
• NCBI Accession #: NP_056045.2
• NCBI GenBank Nucleotide #: NM_015230.3
• UniProt Accession #: Q8WZ64; Q4W5D2; Q7Z2L5; Q96L70; Q96P49; Q9Y4E4
• Molecular Weight: 193,452 Da
• NCBI Official Full Name: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
• NCBI Official Synonym Full Names: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
• NCBI Official Symbol: ARAP2
• NCBI Official Synonym Symbols: PARX; CENTD1
• NCBI Protein Information: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2; cnt-d1; centaurin-delta-1; centaurin, delta 1; Arf and Rho GAP adapter protein 2
• UniProt Protein Name: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ARAP2
• UniProt Synonym Gene Names: CENTD1; KIAA0580; Cnt-d1

Uniprot Description

Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency ().