Western Blot (WB)
(Western Blot: Sample: Recombinant ALOX12B, Mouse.)
Rabbit anti-Mouse Arachidonate-12-Lipoxygenase Polyclonal Antibody | anti-ALOX12B antibody
Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant ALOX12B, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ALOX12B, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded lung tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene can prevent the formation of the epidermal permeability barrier and cause an ichthyosiform phenotype. [provided by RefSeq, Sep 2015]
Uniprot Description
ALOX12B: Converts arachidonic acid to 12R- hydroperoxyeicosatetraenoic acid (12R-HPETE). Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE). NCIE is a non- bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume. Belongs to the lipoxygenase family.
Protein type: EC 1.13.11.-; Lipid Metabolism - arachidonic acid; Oxidoreductase
Chromosomal Location of Human Ortholog: 11 B3|11 42.38 cM
Cellular Component: cytoplasm
Molecular Function: arachidonate 12-lipoxygenase activity; dioxygenase activity; iron ion binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
Biological Process: arachidonic acid metabolic process; ceramide biosynthetic process; fatty acid metabolic process; hepoxilin biosynthetic process; linoleic acid metabolic process; lipid metabolic process; lipoxygenase pathway; positive regulation of MAPK cascade; protein lipidation; sphingolipid metabolic process
Research Articles on ALOX12B
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Product Notes
The ALOX12B alox12b (Catalog #AAA2034091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Arachidonate-12-Lipoxygenase can be used in a range of immunoassay formats including, but not limited to, WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user . Researchers should empirically determine the suitability of the ALOX12B alox12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arachidonate-12-Lipoxygenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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