Rabbit anti-Rat Aquaporin 4 Polyclonal Antibody | anti-AQP4 antibody

Polyclonal Antibody to Aquaporin 4 (AQP4)

Cat. Num. AAA2027206

• Gene Names: Aqp4; Miwc; WCH4; AQP-4
• Reactivity: Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Aquaporin 4; Polyclonal Antibody; Polyclonal Antibody to Aquaporin 4 (AQP4); anti-AQP4 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against AQP4. It has beenselected for its ability to recognize AQP4 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-DS KRTDVTGSVA LAIGFSVAIG HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG AVLAGALYEY VFCPDVELKR RLKEAFSKAA QQTKGSYMEV EDNRSQVETE DLILKPGVVH VIDIDRGDEK
• Sequence Length: 178

• Applicable Applications for anti-AQP4 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Fragment: AQP4 (Asp179~Asp314)
• Organism Species: Rattus norvegicus (Rat)
• Cross Reactivity: Rat
• Conjugate: No Conjugate
• Immunogen: Recombinant AQP4 (Asp179~Asp314) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2044520
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant AQP4, Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Brain Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 395759219
• NCBI GeneID: 25293
• NCBI Accession #: NP_001257487.1
• NCBI GenBank Nucleotide #: NM_001270558.2
• UniProt Accession #: P47863
• Molecular Weight: 28,768 Da
• NCBI Official Full Name: aquaporin-4 isoform 3
• NCBI Official Synonym Full Names: aquaporin 4
• NCBI Official Symbol: Aqp4
• NCBI Official Synonym Symbols: Miwc; WCH4; AQP-4
• NCBI Protein Information: aquaporin-4
• UniProt Protein Name: Aquaporin-4
• Protein Family: Aquaporin
• UniProt Gene Name: Aqp4
• UniProt Synonym Gene Names: AQP-4; MIWC

NCBI Description

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]

Uniprot Description

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 18p13

Cellular Component: basal plasma membrane; basolateral plasma membrane; cell projection membrane; cytoplasm; external side of plasma membrane; integral component of membrane; integral component of plasma membrane; intercellular junction; membrane; plasma membrane; pore complex; protein complex; sarcolemma; T-tubule

Molecular Function: identical protein binding; porin activity; protein binding; transporter activity; water channel activity

Biological Process: carbon dioxide transport; cellular response to estradiol stimulus; cellular response to glucose stimulus; cellular response to interferon-gamma; cellular response to interleukin-1; cellular response to interleukin-6; female pregnancy; hyperosmotic salinity response; multicellular organismal water homeostasis; negative regulation of interleukin-1 beta production; negative regulation of interleukin-6 production; protein homooligomerization; regulation of vascular endothelial growth factor production; renal water absorption; response to glucocorticoid stimulus; response to radiation; sensory perception of sound; transmembrane transport; transport; water homeostasis; water transport