Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AQP5 antibody (MBS9129924) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit AQP5 Polyclonal Antibody | anti-AQP5 antibody
AQP5 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AQP5 antibody (MBS9129924) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AQP5 antibody (MBS9129924) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver injury using AQP5 antibody (MBS9129924) (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver injury using AQP5 antibody (MBS9129924) (40x lens).)
Immunofluoresence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using AQP5 antibody (MBS9129924) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluoresence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using AQP5 antibody (MBS9129924) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluoresence (IF)
(Immunofluorescence analysis of U2OS cells using AQP5 antibody (MBS9129924) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluoresence (IF)
(Immunofluorescence analysis of U2OS cells using AQP5 antibody (MBS9129924) at dilution of 1:100. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
Observed MW: 28 kDa
NCBI Description
Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008]
Uniprot Description
AQP5: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Membrane protein, integral; Transporter, aquaporin family; Channel, misc.; Membrane protein, multi-pass; Transporter
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: apical plasma membrane; basal plasma membrane; endoplasmic reticulum; integral to plasma membrane; microvillus; plasma membrane
Molecular Function: glycerol channel activity; protein binding; water channel activity
Biological Process: camera-type eye morphogenesis; carbon dioxide transport; excretion; glycerol transport; odontogenesis; pancreatic juice secretion; saliva secretion; transmembrane transport; water transport
Research Articles on AQP5
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Product Notes
The AQP5 aqp5 (Catalog #AAA9129924) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AQP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the AQP5 aqp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AQP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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