Rabbit anti-Human, Mouse AQP1 Polyclonal Antibody | anti-AQP1 antibody

AQP1, CT (AQP1, CHIP28, Aquaporin-1, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule) (PE)

Cat. Num. AAA6274620

• Gene Names: AQP1; CO; CHIP28; AQP-CHIP
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AQP1; Polyclonal Antibody; CT (AQP1; CHIP28; Aquaporin-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule) (PE); Water channel protein for red blood cells and kidney proximal tubule; anti-AQP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-AQP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AQP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the C-terminal region of human AQP1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AQP1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AQP1 antibody detected the AQP1 protein (arrow).)

Western Blot (WB)

(AQP1 Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the AQP1 antibody detected the AQP1 protein (arrow).)

Related Product Information for anti-AQP1 antibody

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-AQP1 antibody

Antibodies; Aquaporin

References

Chen, L.M., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 299 (5), R1163-R1174 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Shankardas, J., et al. Mol. Vis. 16, 1538-1548 (2010): Halverson, G.R., et al. Immunohematology 26(1):22-26(2010). Sui, H., et al. Nature 414(6866):872-878(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 37694062
• NCBI GeneID: 358
• NCBI Accession #: NP_932766.1
• NCBI GenBank Nucleotide #: NM_198098.3
• UniProt Accession #: P29972; Q8TBI5; Q8TDC1; B5BU39; E7EM69; E9PC21; F5GY19
• Molecular Weight: 28526 MW
• NCBI Official Full Name: aquaporin-1 isoform 1
• NCBI Official Synonym Full Names: aquaporin 1 (Colton blood group)
• NCBI Official Symbol: AQP1
• NCBI Official Synonym Symbols: CO; CHIP28; AQP-CHIP
• NCBI Protein Information: aquaporin-1
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

NCBI Description

This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq, Aug 2016]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear membrane; integral to plasma membrane; basolateral plasma membrane; brush border membrane; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; ephrin receptor binding; potassium channel activity; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; nitric oxide transporter activity; water channel activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; cellular response to stress; water transport; carbon dioxide transport; negative regulation of caspase activity; pancreatic juice secretion; sensory perception of pain; positive regulation of saliva secretion; odontogenesis; positive regulation of fibroblast proliferation; hyperosmotic salinity response; nitric oxide transport; bicarbonate transport; multicellular organismal water homeostasis; cellular homeostasis; transmembrane transport; potassium ion transport; lipid digestion; lateral ventricle development; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; renal water homeostasis; cell volume homeostasis; negative regulation of apoptosis

Disease: Blood Group--colton