Rabbit APTX Polyclonal Antibody | anti-APTX antibody

APTX Antibody

Cat. Num. AAA9414531

• Gene Names: APTX; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: APTX; Polyclonal Antibody; APTX Antibody; AOA; AOA1; AXA1; EAOH; EOAHA; anti-APTX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total APTX protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 356

• Applicable Applications for anti-APTX antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human APTX.
• Target Name: APTX
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using APTX antibody.)

Related Product Information for anti-APTX antibody

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

Product Categories/Family for anti-APTX antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 305410829
• NCBI GeneID: 54840
• NCBI Accession #: NP_001182177.1
• NCBI GenBank Nucleotide #: NM_001195248.1
• UniProt Accession #: Q7Z2E3; Q0P662; Q5T781; Q5T782; Q5T784; Q6JV81; Q6JV82; Q6JV85; Q7Z2F3; A8MTN4; D3DRK9; D3DRL0
• Molecular Weight: 21,389 Da
• NCBI Official Full Name: aprataxin isoform e
• NCBI Official Synonym Full Names: aprataxin
• NCBI Official Symbol: APTX
• NCBI Official Synonym Symbols: AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
• NCBI Protein Information: aprataxin
• UniProt Protein Name: Aprataxin
• Protein Family: Aprataxin
• UniProt Gene Name: APTX
• UniProt Synonym Gene Names: AXA1; FHA-HIT
• UniProt Entry Name: APTX_HUMAN

NCBI Description

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

APTX: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA). AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA repair, damage; EC 3.-.-.-; Phosphatase (non-protein); Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; nucleolus; chromatin; nucleus

Molecular Function: protein binding; DNA 5'-adenosine monophosphate hydrolase activity; phosphoglycolate phosphatase activity; polynucleotide 3'-phosphatase activity; metal ion binding; double-stranded DNA binding; double-stranded RNA binding; damaged DNA binding; protein N-terminus binding; phosphoprotein binding; chromatin binding

Biological Process: single strand break repair; response to hydrogen peroxide; dephosphorylation; double-strand break repair; regulation of protein stability; DNA catabolic process, exonucleolytic; response to DNA damage stimulus; DNA ligation

Disease: Ataxia, Early-onset, With Oculomotor Apraxia And Hypoalbuminemia