Rabbit anti-Human APPBP1 Polyclonal Antibody | anti-APPBP1 antibody

APPBP1, CT (NEDD8-activating Enzyme E1 Regulatory Subunit, Amyloid Protein-binding Protein 1, Amyloid beta Precursor Protein-binding Protein 1, 59kD, APP-BP1, Proto-oncogene Protein 1, NAE1, HPP1) (Azide free) (HRP)

Cat. Num. AAA6462991

• Gene Names: NAE1; HPP1; ula-1; APPBP1; A-116A10.1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: APPBP1; Polyclonal Antibody; CT (NEDD8-activating Enzyme E1 Regulatory Subunit; Amyloid Protein-binding Protein 1; Amyloid beta Precursor Protein-binding Protein 1; 59kD; APP-BP1; Proto-oncogene Protein 1; NAE1; HPP1) (Azide free) (HRP); anti-APPBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APPBP1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-APPBP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human APPBP1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-APPBP1 Antibody (C-term) in mouse brain tissue lysates (35ug/lane). APPBP1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of APP-BP1(arrow) using rabbit polyclonal APP-BP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-APPBP1 antibody

Antibodies; Binding Proteins

References

1. Chen, Y., J. Cell Biol. 163(1), 27-33 (2003). 2. Chen, Y., J. Neurochem. 85(3), 801-809 (2003). 3. Walden, H., Nature 422(6929), 330-334 (2003). 4. Chow, N., J. Biol. Chem. 271(19), 11339-11346 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4502169
• NCBI GeneID: 8883
• NCBI Accession #: NP_003896
• NCBI GenBank Nucleotide #: NM_003905.3
• UniProt Accession #: Q13564; A6NCK0; A6NFN4; A8MU28; B2R700; B3KUP9
• Molecular Weight: 62.7kDa (557aa)
• NCBI Official Full Name: NEDD8-activating enzyme E1 regulatory subunit isoform a
• NCBI Official Synonym Full Names: NEDD8 activating enzyme E1 subunit 1
• NCBI Official Symbol: NAE1
• NCBI Official Synonym Symbols: HPP1; ula-1; APPBP1; A-116A10.1
• NCBI Protein Information: NEDD8-activating enzyme E1 regulatory subunit
• UniProt Protein Name: NEDD8-activating enzyme E1 regulatory subunit
• UniProt Gene Name: NAE1
• UniProt Synonym Gene Names: APPBP1; APP-BP1
• UniProt Entry Name: ULA1_HUMAN

NCBI Description

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

APPBP1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding

Biological Process: regulation of apoptosis; neuron apoptosis; protein neddylation; regulation of neuron apoptosis; mitotic cell cycle DNA replication checkpoint; signal transduction