Goat APOLIPOPROTEIN L1 Polyclonal Antibody | anti-APOL1 antibody

GOAT ANTI HUMAN APOLIPOPROTEIN L1 (C-TERMINAL)

Cat. Num. AAA223556

• Gene Names: APOL1; APOL; APO-L; FSGS4; APOL-I
• Applications: ELISA, Western Blot
• Synonyms: APOLIPOPROTEIN L1; Polyclonal Antibody; GOAT ANTI HUMAN APOLIPOPROTEIN L1 (C-TERMINAL); anti-APOL1 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the C-Terminal (CT) region of human Apolipoprotein L1 (APOL1), a secreted high density lipoprotein and member of the apolipoprotein L family, which interacts with apolipoprotein A-I (APOA1) in plasma. Two isoforms of APOL1 have been identified, which may play a role in lipid exchange and transport throughout the body, and possibly in the reverse transport of cholesterol from peripheral cells to the liver. This item is expected to recognise both reported isoforms of human APOL1.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 398

• Applicable Applications for anti-APOL1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 45kDa in human brain (frontal cortex) cell lysates; ELISA: Minimum Dilution: 1/8000; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 3.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Synthetic peptide C-NNNYKILQADQE from within the C-Terminal region of human APOL1 (NP_003652.2; NP_663318.1).; Buffer Solution
• Antiserum Preparation: Antiserum to human APOL1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot of human frontal cortex lysate probed with Goat anti human apolipoprotein L1 (MBS223556))

Related Product Information for anti-APOL1 antibody

MBS223556 specifically recognises an epitope within the C-Terminal (CT) region of human Apolipoprotein L1 (APOL1), a secreted high density lipoprotein and member of the apolipoprotein L family, which interacts with apolipoprotein A-I (APOA1) in plasma. Two isoforms of APOL1 have been identified, which may play a role in lipid exchange and transport throughout the body, and possibly in the reverse transport of cholesterol from peripheral cells to the liver. MBS223556 is expected to recognise both reported isoforms of human APOL1.

NCBI and Uniprot Product Information

• NCBI GI #: 211938440
• NCBI GeneID: 8542
• NCBI Accession #: NP_001130012.1
• NCBI GenBank Nucleotide #: NM_001136540.1
• UniProt Accession #: O14791; O60804; Q5R3P7; Q5R3P8; Q96AB8; Q96PM4; Q9BQ03; A5PLQ4; B4DU12; E9PF24
• Molecular Weight: 42,158 Da
• NCBI Official Full Name: apolipoprotein L1 isoform a
• NCBI Official Synonym Full Names: apolipoprotein L, 1
• NCBI Official Symbol: APOL1
• NCBI Official Synonym Symbols: APOL; APO-L; FSGS4; APOL-I
• NCBI Protein Information: apolipoprotein L1
• UniProt Protein Name: Apolipoprotein L1
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOL1
• UniProt Synonym Gene Names: APOL; Apo-L; ApoL; ApoL-I
• UniProt Entry Name: APOL1_HUMAN

NCBI Description

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Channel, chloride; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; extracellular region; intrinsic to membrane

Molecular Function: chloride channel activity; protein binding; lipid binding

Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; killing of cells of another organism; cytolysis; innate immune response; lipoprotein metabolic process; chloride transport; lipid transport

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To