Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Guinea Pig anti-Rabbit Apolipoprotein A1 (APOA1) Polyclonal Antibody | anti-APOA1 antibody
Polyclonal Antibody to Apolipoprotein A1 (APOA1)
Reactivity
Rabbit
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Apolipoprotein A1 (APOA1); Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein A1 (APOA1); anti-APOA1 antibody
Host
Guinea Pig
Reactivity
Rabbit
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against APOA1. It has been selected for its ability to recognize APOA1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGS-DEPRSS WDKIKDFATV YVDTVKDSGR EYVAQFEASA FGKQLNLKLL DNWDSLSSTV SKLQEQLGPV TQEFWDNLEK ETEGLREEMN KDLQEVRQKV QPYLDEFQKK WQEEVERYRQ KVEPLGAELR ESARQKLTEL QEKLSPLAEE LRDSARTHVD TLRTKLAPYS NELQQRLAAR LESIKEGGGA SLAEYQAKAR EHLSVLSEKA RPALEDLRQG LLPVLESFKA SVQNVLDEAT KKLNT
MGHHHHHHSGS-DEPRSS WDKIKDFATV YVDTVKDSGR EYVAQFEASA FGKQLNLKLL DNWDSLSSTV SKLQEQLGPV TQEFWDNLEK ETEGLREEMN KDLQEVRQKV QPYLDEFQKK WQEEVERYRQ KVEPLGAELR ESARQKLTEL QEKLSPLAEE LRDSARTHVD TLRTKLAPYS NELQQRLAAR LESIKEGGGA SLAEYQAKAR EHLSVLSEKA RPALEDLRQG LLPVLESFKA SVQNVLDEAT KKLNT
Sequence Length
67
Applicable Applications for anti-APOA1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant APOA1 (Asp25~Thr265) expressed in E.coli.
Cross Reactivity
Rabbit
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,591 Da
NCBI Official Full Name
apolipoprotein A-I preproprotein
NCBI Official Symbol
APOA1
NCBI Protein Information
apolipoprotein A-I
UniProt Protein Name
Apolipoprotein A-I
Protein Family
UniProt Gene Name
APOA1
UniProt Synonym Gene Names
Apo-AI; ApoA-I; ProapoA-I
Uniprot Description
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Research Articles on APOA1
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Product Notes
The APOA1 apoa1 (Catalog #AAA2005409) is an Antibody produced from Guinea Pig and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apolipoprotein A1 (APOA1) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein A1 (APOA1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the APOA1 apoa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-DEPRSS WDKIKDFATV YVDTVKDSGR EYVAQFEASA FGKQLNLKLL DNWDSLSSTV SKLQEQLGPV TQEFWDNLEK ETEGLREEMN KDLQEVRQKV QPYLDEFQKK WQEEVERYRQ KVEPLGAELR ESARQKLTEL QEKLSPLAEE LRDSARTHVD TLRTKLAPYS NELQQRLAAR LESIKEGGGA SLAEYQAKAR EHLSVLSEKA RPALEDLRQG LLPVLESFKA SVQNVLDEAT KKLNT. It is sometimes possible for the material contained within the vial of "Apolipoprotein A1 (APOA1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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