Rabbit anti-Human APOC2 Polyclonal Antibody | anti-APOC2 antibody

APOC2, ID (Apolipoprotein C-II, Apo-CII, ApoC-II, APC2) (PE)

Cat. Num. AAA6463730

• Gene Names: APOC2; APO-CII; APOC-II
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: APOC2; Polyclonal Antibody; ID (Apolipoprotein C-II; Apo-CII; ApoC-II; APC2) (PE); anti-APOC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APOC2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-APOC2 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human APOC2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(APOC2 Antibody (Center) western blot analysis in HL-60(lane 1),A549(lane 2) cell line lysates (35ug/lane).This demonstrates the APOC2 antibody detected the APOC2 protein (arrow).)

Immunohistochemistry (IHC)

(APOC2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APOC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-APOC2 antibody

Antibodies; Lipoproteins

References

1. Bahri, R., Lipids Health Dis 7, 46 (2008). 2. Hegele, R.A., Dis. Markers 9(2), 73-80 (1991). 3. Bengtsson-Olivecrona, G., Eur. J. Biochem. 192(2), 515-521 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 32130518
• NCBI GeneID: 344
• NCBI Accession #: NP_000474
• NCBI GenBank Nucleotide #: NM_000483.4
• UniProt Accession #: P02655
• Molecular Weight: 11kDa
• NCBI Official Full Name: apolipoprotein C-II
• NCBI Official Synonym Full Names: apolipoprotein C2
• NCBI Official Symbol: APOC2
• NCBI Official Synonym Symbols: APO-CII; APOC-II
• NCBI Protein Information: apolipoprotein C-II
• UniProt Protein Name: Apolipoprotein C-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC2
• UniProt Synonym Gene Names: APC2; Apo-CII; ApoC-II
• UniProt Entry Name: APOC2_HUMAN

NCBI Description

This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family. The protein is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. This gene is present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally occurring read-through transcription exists between this gene and the neighboring upstream apolipoprotein C-IV (APOC4) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

APOC2: Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly. Defects in APOC2 are the cause of hyperlipoproteinemia type 1B (HLPP1B). It is an autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Belongs to the apolipoprotein C2 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; chylomicron; early endosome; extracellular region

Molecular Function: protein homodimerization activity; phospholipase binding; lipase inhibitor activity; lipid binding; phospholipase activator activity

Biological Process: positive regulation of fatty acid biosynthetic process; phototransduction, visible light; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; negative regulation of cholesterol transport; negative regulation of catalytic activity; positive regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; lipoprotein metabolic process; cholesterol efflux; negative regulation of lipid metabolic process; retinoid metabolic process; lipid catabolic process

Disease: Apolipoprotein C-ii Deficiency