anti-Human APOBEC4 Polyclonal Antibody | anti-APOBEC4 antibody
Goat Anti-APOBEC4
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
APOBEC4; Polyclonal Antibody; Goat Anti-APOBEC4; Apolipoprotein B mRNA editing enzyme; catalytic polypeptide-like 4 (putative); C1orf169; MGC26594; OTTHUMP00000060680; putative C->U-editing enzyme APOBEC-4; anti-APOBEC4 antibody
Host
Host: Goat; Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human APOBEC4.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-APOBEC4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: 1:32,000. Western Blot: 0.03ug/ml. A band is detected at ~48kD band in Human Bone Marrow lysates. Optimal dilutions to be determined by the researcher.
Immunogen
A synthetic peptide from the C-terminus of aa-QTKRNPNTKAQE.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See MBS653697. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The APOBEC4 (Catalog #AAA6120688) is an Antibody produced from Host: Goat; Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-APOBEC4 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOBEC4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Peptide ELISA: 1:32,000. Western Blot: 0.03ug/ml. A band is detected at ~48kD band in Human Bone Marrow lysates. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the APOBEC4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOBEC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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