Western Blot (WB)
(APOBEC3A Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the APOBEC3A antibody detected the APOBEC3A protein (arrow).)
Rabbit anti-Human APOBEC3A Polyclonal Antibody | anti-APOBEC3A antibody
APOBEC3A Antibody (N-term)
Western Blot (WB)
(APOBEC3A Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the APOBEC3A antibody detected the APOBEC3A protein (arrow).)
Western Blot (WB)
(APOBEC3A Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the APOBEC3A antibody detected the APOBEC3A protein (arrow).)
family. It is one of seven related genes or pseudogenes found in a
cluster, thought to result from gene duplication, on chromosome 22.
Members of the cluster encode proteins that are structurally and
functionally related to the C to U RNA-editing cytidine deaminase
APOBEC1. The protein encoded by this gene lacks the zinc binding
activity of other family members. The protein plays a role in
immunity, by restricting transmission of foreign DNA such as
viruses. One mechanism of foreign DNA restriction is deamination of
foreign double-stranded DNA cytidines to uridines, which leads to
DNA degradation. However, other mechanisms are also thought to be
involved, as anti-viral effect is not dependent on deaminase
activity. One allele of this gene results from the deletion of
approximately 29.5 kb of sequence between this gene, APOBEC3A, and
the adjacent gene APOBEC3B. The breakpoints of the deletion are
within the two genes, so the deletion allele is predicted to have
the promoter and coding region of APOBEC3A, but the 3' UTR of
APOBEC3B.
Berger, A., et al. J. Biol. Chem. 285(16):12248-12254(2010)
Stenglein, M.D., et al. Nat. Struct. Mol. Biol. 17(2):222-229(2010)
Abe, H., et al. Hepatol. Res. 39(12):1159-1168(2009)
Prochnow, C., et al. Sci. China, C, Life Sci. 52(10):893-902(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. The protein encoded by this gene is the same as that encoded by APOBEC3A; however, this gene is a hybrid gene that results from the deletion of approximately 29.5 kb of sequence between the APOBEC3A gene and the adjacent gene APOBEC3B. The breakpoints of the deletion are within the two genes, so the deletion hybrid is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. [provided by RefSeq, Jul 2012]
Uniprot Description
APOBEC3A: a single-stranded DNA cytidine deaminase involved in foreign DNA clearance with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic C-to-U hypermutation in nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Interacts with AGO2. Interacts with TRIB3. Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia. Up-regulated by interferon and CpG single-stranded DNA (at protein level). Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: EC 3.5.4.-; Hydrolase
Chromosomal Location of Human Ortholog: 22q13.1-q13.2
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; zinc ion binding; deoxycytidine deaminase activity; cytidine deaminase activity
Biological Process: negative regulation of viral genome replication; innate immune response; cytidine deamination; defense response to virus
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Product Notes
The APOBEC3A apobec3a (Catalog #AAA9209397) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 20-49. The APOBEC3A Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOBEC3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the APOBEC3A apobec3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOBEC3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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