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Western Blot (WB) (Anti-APOA4 Antibody at 1:8000 dilution + human plasma lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human APOA4 Polyclonal Antibody | anti-APOA4 antibody

APOA4 Antibody

Reactivity
Human
Applications
Western Blot
Synonyms
APOA4; Polyclonal Antibody; APOA4 Antibody; Purified Rabbit Polyclonal Antibody (Pab); Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4; anti-APOA4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This APOA4 antibody is generated from a rabbit immunized with a recombinant protein.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
396
Applicable Applications for anti-APOA4 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:8000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-APOA4 Antibody at 1:8000 dilution + human plasma lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-APOA4 Antibody at 1:8000 dilution + human plasma lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-APOA4 antibody
May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Product Categories/Family for anti-APOA4 antibody
References
Karathanasis S.K.,et al.Biochemistry 25:3962-3970(1986). Karathanasis S.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:8457-8461(1986). Elshourbagy N.A.,et al.J. Biol. Chem. 262:7973-7981(1987). Yang C.,et al.Biochim. Biophys. Acta 1002:231-237(1989). Fullerton S.M.,et al.Hum. Genet. 115:36-56(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
337
UniProt Accession #
Molecular Weight
45399
NCBI Official Full Name
Apolipoprotein A-IV
NCBI Official Synonym Full Names
apolipoprotein A-IV
NCBI Official Symbol
APOA4
NCBI Protein Information
apolipoprotein A-IV
UniProt Protein Name
Apolipoprotein A-IV
Protein Family
UniProt Gene Name
APOA4
UniProt Synonym Gene Names
Apo-AIV; ApoA-IV
UniProt Entry Name
APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Endoplasmic reticulum; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: lipid transporter activity; antioxidant activity; protein binding; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: phototransduction, visible light; removal of superoxide radicals; lipid homeostasis; protein-lipid complex assembly; phosphatidylcholine metabolic process; leukocyte adhesion; phospholipid efflux; regulation of cholesterol absorption; hydrogen peroxide catabolic process; triacylglycerol catabolic process; retinoid metabolic process; positive regulation of fatty acid biosynthetic process; cholesterol metabolic process; multicellular organismal lipid catabolic process; neurite regeneration; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; cholesterol homeostasis; reverse cholesterol transport; innate immune response in mucosa; response to lipid hydroperoxide; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport

Research Articles on APOA4

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Product Notes

The APOA4 apoa4 (Catalog #AAA9216379) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APOA4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:8000. Researchers should empirically determine the suitability of the APOA4 apoa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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