Goat APOA4 Polyclonal Antibody | anti-APOA4 antibody

Goat anti-APOA4 Antibody

Cat. Num. AAA420479

• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: APOA4; Polyclonal Antibody; Goat anti-APOA4 Antibody; apolipoprotein A-IV; MGC142154; MGC142156; APOA4 antibody; apolipoprotein A-IV antibody; MGC142154 antibody; MGC142156 antibody; anti-APOA4 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KEKESQDKTLSLP
• Sequence Length: 396

• Applicable Applications for anti-APOA4 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Intestine shows specific staining of cell groups in the Lamina propria. Recommended concentration, 2-4ug/ml.; Western Blot: Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 45.4kDa according to NP_000473.2). Recommended concentration: 0.01-0.03ug/ml.
• Immunogen: Peptide with sequence C-KEKESQDKTLSLP, from the internal region (near the C Terminus) of the protein sequence according to NP_000473.2.
• Epitope: Internal region (near the C Terminus)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.01ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((2ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 71773110
• NCBI GeneID: 337
• NCBI Accession #: NP_000473.2
• NCBI GenBank Nucleotide #: NM_000482.3
• Molecular Weight: 45,399 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A4
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding; Cell adhesion; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: cell surface; chylomicron; cytosol; early endosome; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: antioxidant activity; cholesterol binding; cholesterol transporter activity; copper ion binding; lipid binding; lipid transporter activity; phosphatidylcholine binding; protein binding; protein homodimerization activity

Biological Process: cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; hydrogen peroxide catabolic process; innate immune response in mucosa; leukocyte adhesion; lipid homeostasis; lipid transport; lipoprotein biosynthetic process; lipoprotein metabolic process; multicellular organismal lipid catabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; protein-lipid complex assembly; regulation of cholesterol absorption; regulation of cholesterol transport; removal of superoxide radicals; response to lipid hydroperoxide; retinoid metabolic process; reverse cholesterol transport