Rabbit ApoA4 Polyclonal Antibody | anti-APOA4 antibody

ApoA4 Antibody

Cat. Num. AAA150126

• Reactivity: Human, Chicken
• Applications: ELISA, Western Blot
• Purity: ApoA4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ApoA4; Polyclonal Antibody; ApoA4 Antibody; Apolipoprotein A-IV; Apolipoprotein A4; Apo-AIV; apolipoprotein A-IV; anti-APOA4 antibody

• Host: Rabbit
• Reactivity: Human, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ApoA4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 396

• Applicable Applications for anti-APOA4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ApoA4 antibody can be used for detection of ApoA4 by Western blot at 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ApoA4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ApoA4.
• Buffer: ApoA4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ApoA4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ApoA4 in human testis tissue lysate with ApoA4 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-APOA4 antibody

ApoA4 Antibody: Apolipoprotein A4 (also known as ApoA-IV) is a plasma protein that is an O-linked glycoprotein after proteolytic processing. ApoA4 is a major constituent of HDL and it also can be found on the surface of newly synthesized chylomicrons. The physiological function of ApoA4 is not completely identified so far, but evidence from several in vitro studies indicate that this apolipoprotein is involved in several steps of the reverse cholesterol transport pathway, which transports cholesterol from peripheral cells back to the liver.

NCBI and Uniprot Product Information

• NCBI GI #: 71773110
• NCBI GeneID: 337
• NCBI Accession #: AAA51744
• NCBI GenBank Nucleotide #: NM_000482.3
• UniProt Accession #: P06727; Q14CW8; Q6Q787; A8MSL6
• Molecular Weight: 45,399 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Endoplasmic reticulum; Cell adhesion; Secreted, signal peptide; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: lipid transporter activity; antioxidant activity; protein binding; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: cholesterol metabolic process; phototransduction, visible light; positive regulation of fatty acid biosynthetic process; multicellular organismal lipid catabolic process; removal of superoxide radicals; lipid homeostasis; neurite regeneration; protein-lipid complex assembly; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; cholesterol homeostasis; leukocyte adhesion; reverse cholesterol transport; phospholipid efflux; innate immune response in mucosa; regulation of cholesterol absorption; hydrogen peroxide catabolic process; response to lipid hydroperoxide; positive regulation of lipoprotein lipase activity; retinoid metabolic process; regulation of cholesterol transport