Rabbit anti-Human, Mouse Apg7 / ATG7 Polyclonal Antibody | anti-Apg7 antibody

Rabbit Polyclonal to Human Apg7 / ATG7

Cat. Num. AAA241919

• Gene Names: ATG7; GSA7; APG7L; APG7-LIKE
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: Apg7 / ATG7; Polyclonal Antibody; Rabbit Polyclonal to Human Apg7 / ATG7; Anti-Apg7 / ATG7 Antibody (N-Terminus) IHC-plus; ATG7; Apg7; APG7L; APG7-LIKE; Autophagy related 7; Autophagy-related protein 7; HAGP7; GSA7; Human Apg7; anti-Apg7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: 17 amino acid peptide from near the amino terminus of human APG7.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 703

• Applicable Applications for anti-Apg7 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human ATG7.
• Immunogen Type: Synthetic peptide
• Immunogen: Apg7 / ATG7 antibody was raised against synthetic peptide from human ATG7.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-ATG7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453668
• NCBI GeneID: 10533
• NCBI Accession #: NP_006386.1
• NCBI GenBank Nucleotide #: NM_006395.2
• UniProt Accession #: O95352; Q7L8L0; Q9BWP2; Q9UFH4; B4E170; E9PB95
• Molecular Weight: 68,618 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform a
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: GSA7; APG7L; APG7-LIKE
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7L; APG7-like; hAGP7
• UniProt Entry Name: ATG7_HUMAN

NCBI Description

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

Uniprot Description

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; axoneme; cytosol

Molecular Function: protein binding; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; ubiquitin activating enzyme activity; transcription factor binding

Biological Process: positive regulation of apoptosis; regulation of protein ubiquitination; protein ubiquitination; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; protein amino acid lipidation; protein catabolic process; positive regulation of autophagy; amino acid metabolic process; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; protein modification process; C-terminal protein lipidation; cellular response to starvation; liver development; neurological system process; cellular response to nitrogen starvation; protein modification by small protein conjugation; positive regulation of protein modification process; mitochondrion degradation; positive regulation of protein catabolic process; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis