Rabbit AP2A2 Polyclonal Antibody | anti-Ap2a2 antibody

Anti-AP2A2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102371

• Gene Names: AP2A2; HIP9; HYPJ; ADTAB; HIP-9; CLAPA2
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: AP2A2; Polyclonal Antibody; Anti-AP2A2 Antibody; Rabbit Polyclonal; Ap2a2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-ADTAB Antibody; Anti-CLAPA2 Antibody; Anti-HIP9 Antibody; Anti-HYPJ Antibody; Anti-KIAA0899 Antibody; anti-Ap2a2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human AP2A2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Ap2a2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Ap2a2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Ap2a2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-AP2A2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:104 kDaObserved band size:120 kDa)

Related Product Information for anti-Ap2a2 antibody

AP-2 is a stable protein complex comprising 4 domains, which are termed alpha, beta, mu, and sigma. AP2A1 and AP2A2 are homologous human genes that encode the alpha subunit of the AP-2 complex. The AP2A2 transcript is expressed in a broad range of CNS cell lineages. At the tissue level, both AP2A1 and AP2A2 are highly expressed in human cerebellum, but AP2A2 is expressed at lower levels in the cerebral cortex. AP2A2 (Adaptor Related Protein Complex 2 Subunit Alpha 2, also known as HIP9; HYPJ; ADTAB; HIP-9; CLAPA2) is located on human chromosome 11p15.5. The AP2A2 protein is ubiquitously expressed in spleen, brain and other tissues. Structurally, the encoded AP2A2 protein is reported to be 103960 Da in mass.

References

Nelson PT, et al. (2020) The muc6/ap2a2 locus and its relevance to alzheimer's disease: A review. J Neuropathol Exp Neurol 79 (6): 568-584.

NCBI and Uniprot Product Information

• NCBI GI #: 12643300
• NCBI GeneID: 161
• UniProt Accession #: O94973; O75403; Q53ET1; Q96SI8
• Molecular Weight: 73,128 Da
• NCBI Official Full Name: AP-2 complex subunit alpha-2
• NCBI Official Synonym Full Names: adaptor related protein complex 2 subunit alpha 2
• NCBI Official Symbol: AP2A2
• NCBI Official Synonym Symbols: HIP9; HYPJ; ADTAB; HIP-9; CLAPA2
• NCBI Protein Information: AP-2 complex subunit alpha-2
• UniProt Protein Name: AP-2 complex subunit alpha-2
• Protein Family: AP-2 complex
• UniProt Gene Name: AP2A2
• UniProt Synonym Gene Names: ADTAB; CLAPA2; HIP9; HYPJ; KIAA0899; HIP-9

NCBI Description

The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016]

Uniprot Description

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif ().