Rabbit anti-Human AP2A1 Polyclonal Antibody | anti-AP2A1 antibody

AP2A1, CT (AP2A1, ADTAA, CLAPA1, AP-2 complex subunit alpha-1, 100kD coated vesicle protein A, Adapter-related protein complex 2 alpha-1 subunit, Adaptor protein complex AP-2 subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin assembly protein comp

Cat. Num. AAA6274061

• Gene Names: AP2A1; ADTAA; CLAPA1; AP2-ALPHA
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AP2A1; Polyclonal Antibody; CT (AP2A1; ADTAA; CLAPA1; AP-2 complex subunit alpha-1; 100kD coated vesicle protein A; Adapter-related protein complex 2 alpha-1 subunit; Adaptor protein complex AP-2 subunit alpha-1; Alpha-adaptin A; Alpha1-adaptin; Clathrin assembly protein comp; Clathrin assembly protein complex 2 alpha-A large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; anti-AP2A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AP2A1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: AP2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-735 amino acids from the C-terminal region of human AP2A1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AP2A1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AP2A1 antibody detected the AP2A1 protein (arrow).)

Immunohistochemistry (IHC)

(AP2A1 Antibody (C-term) (AP13905b)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP2A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-AP2A1 antibody

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

Product Categories/Family for anti-AP2A1 antibody

Antibodies; Clathrin

References

Mitchell, R.S., et al. J. Virol. 82(16):7758-7767(2008). Kitagawa, Y., et al. Virology 373(1):171-180(2008). Stove, V., et al. J. Virol. 79(17):11422-11433(2005). Lu, W., et al. Neuron 47(3):407-421(2005). Pope, S.N., et al. J. Steroid Biochem. Mol. Biol. 94 (1-3), 203-208 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 19913416
• NCBI GeneID: 160
• NCBI Accession #: NP_570603.2; NP_055018.2
• NCBI GenBank Nucleotide #: NM_130787.2
• UniProt Accession #: O95782; Q96CI7; Q96PP6; Q96PP7; Q9H070; Q8N9K4
• Molecular Weight: 107,546 Da
• NCBI Official Full Name: AP-2 complex subunit alpha-1 isoform 2
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, alpha 1 subunit
• NCBI Official Symbol: AP2A1
• NCBI Official Synonym Symbols: ADTAA; CLAPA1; AP2-ALPHA
• NCBI Protein Information: AP-2 complex subunit alpha-1; alpha1-adaptin; alpha-adaptin A; adaptin, alpha A; 100 kDa coated vesicle protein A; adaptor protein complex AP-2 subunit alpha-1; adapter-related protein complex 2 alpha-1 subunit; clathrin assembly protein complex 2 alpha-A
• UniProt Protein Name: AP-2 complex subunit alpha-1
• Protein Family: AP-2 complex
• UniProt Gene Name: AP2A1
• UniProt Synonym Gene Names: ADTAA; CLAPA1
• UniProt Entry Name: AP2A1_HUMAN

NCBI Description

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq]