Rabbit anti-Human, Mouse AP1S3 Polyclonal Antibody | anti-AP1S3 antibody

AP1S3, NT (AP1S3, AP-1 complex subunit sigma-3, Adapter-related protein complex 1 sigma-1C subunit, Adaptor protein complex AP-1 sigma-1C subunit, Clathrin assembly protein complex 1 sigma-1C small chain, Golgi adaptor HA1/AP1 adaptin sigma-1C subunit, Si

Cat. Num. AAA6274059

• Gene Names: AP1S3; PSORS15
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AP1S3; Polyclonal Antibody; NT (AP1S3; AP-1 complex subunit sigma-3; Adapter-related protein complex 1 sigma-1C subunit; Adaptor protein complex AP-1 sigma-1C subunit; Clathrin assembly protein complex 1 sigma-1C small chain; Golgi adaptor HA1/AP1 adaptin sigma-1C subunit; Si; Sigma 1C subunit of AP-1 clathrin; Sigma-adaptin 1C; Sigma1C-adaptin; anti-AP1S3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-AP1S3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: AP1S3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human AP1S3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AP1S3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the AP1S3 antibody detected the AP1S3 protein (arrow).)

Immunohistochemistry (IHC)

(AP1S3 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP1S3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-AP1S3 antibody

Antibodies; Clathrin

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Roeth, J.F., et al. J. Cell Biol. 167(5):903-913(2004). Hirst, J., et al. Mol. Biol. Cell 12(11):3573-3588(2001). Boehm, M., et al. Mol. Biol. Cell 12(10):2907-2920(2001). Kirchhausen, T. Nat. Rev. Mol. Cell Biol. 1(3):187-198(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 88703051
• NCBI GeneID: 130340
• NCBI Accession #: NP_001034658.1
• NCBI GenBank Nucleotide #: NM_001039569.1
• UniProt Accession #: Q96PC3; Q8WTY1; Q96DD1; B4DQZ1
• Molecular Weight: 12,655 Da
• NCBI Official Full Name: AP-1 complex subunit sigma-3
• NCBI Official Synonym Full Names: adaptor related protein complex 1 sigma 3 subunit
• NCBI Official Symbol: AP1S3
• NCBI Official Synonym Symbols: PSORS15
• NCBI Protein Information: AP-1 complex subunit sigma-3
• UniProt Protein Name: AP-1 complex subunit sigma-3
• Protein Family: AP-1 complex
• UniProt Gene Name: AP1S3
• UniProt Entry Name: AP1S3_HUMAN

NCBI Description

This gene encodes a member of the adaptor-related protein complex 1, sigma subunit genes. The encoded protein is a component of adaptor protein complex 1 (AP-1), one of the AP complexes involved in claathrin-mediated vesicular transport from the Golgi or endosomes. Disruption of the pathway for display of HIV-1 antigens, which prevents recognition of the virus by cytotoxic T cells, has been shown to involve the AP-1 complex (PMID: 15569716). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

AP1S3: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes small subunit family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: coated pit; cytoplasmic vesicle membrane; cytosol; Golgi membrane; lysosomal membrane; membrane coat; trans-Golgi network membrane

Molecular Function: protein binding; protein transporter activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; protein targeting; regulation of defense response to virus by virus; viral reproduction

Disease: Psoriasis 15, Pustular, Susceptibility To