Western Blot (WB)
(Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody (Center). MBS9207533 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit AP1M1 Polyclonal Antibody | anti-AP1M1 antibody
AP1M1 Antibody (Center)
Western Blot (WB)
(Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody (Center). MBS9207533 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody (Center). MBS9207533 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Immunohistochemistry (IHC)
(AP1M1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(AP1M1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of AP1M1 Antibody (Center) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of AP1M1 Antibody (Center) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
the trans-Golgi network clathrin-associated protein complex AP-1.
The other components of this complex are beta-prime-adaptin,
gamma-adaptin, and the small chain AP1S1. This complex is located
at the Golgi vesicle and links clathrin to receptors in coated
vesicles. These vesicles are involved in endocytosis and Golgi
processing. Alternatively spliced transcript variants encoding
distinct protein isoforms have been found for this gene. [provided
by RefSeq].
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Noviello, C.M., et al. J. Virol. 82(3):1249-1258(2008)
Medigeshi, G.R., et al. Traffic 9(1):121-132(2008)
Roeth, J.F., et al. J. Cell Biol. 167(5):903-913(2004)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
AP1M1: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes medium subunit family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 19p13.12
Cellular Component: Golgi membrane; clathrin adaptor complex; cytoplasmic vesicle membrane; membrane; lysosomal membrane; clathrin coated vesicle membrane; trans-Golgi network membrane; cytosol
Molecular Function: protein binding
Biological Process: intracellular protein transport; viral reproduction; melanosome organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus
Research Articles on AP1M1
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Product Notes
The AP1M1 ap1m1 (Catalog #AAA9207533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 199-227. The AP1M1 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's AP1M1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the AP1M1 ap1m1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP1M1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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