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Western Blot (WB) (Western blot detection of c-Jun in Jurkat and Hela cell lysates using c-Jun rabbit pAb (1:500 diluted).Predicted band size:38kDa.Observed band size:48kDa.)

Rabbit anti-Human AP-1 Polyclonal Antibody | anti-AP-1 antibody

Anti-AP-1 Rabbit pAb

Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
Purity
Affinity Purified
Synonyms
AP-1; Polyclonal Antibody; Anti-AP-1 Rabbit pAb; HSPA1B; Activator protein 1; AP 1; AP1; cJun; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN; Jun oncogene; JUN protein; Jun proto oncogene; JUN_HUMAN; JUNC; Oncogene JUN; p39; Proto oncogene c jun; Proto oncogene cJun; Proto-oncogene c-jun; Transcription Factor AP 1; Transcription factor AP-1; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; V jun sarcoma virus 17 oncogene homolog (avian); V jun sarcoma virus 17 oncogene homolog; V-jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; anti-AP-1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Sequence Length
331
Applicable Applications for anti-AP-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
IF: 1:50-200
WB: 1:500-1:2000
IHC: 1:100-1:300
IP: 2-5 ug:mg lysate
ELISA: 1:20000
Immunogen
Synthesized peptide derived from AP-1. at AA range: 30-110.
Subcellular Location
Nucleus

Western Blot (WB)

(Western blot detection of c-Jun in Jurkat and Hela cell lysates using c-Jun rabbit pAb (1:500 diluted).Predicted band size:38kDa.Observed band size:48kDa.)

Western Blot (WB) (Western blot detection of c-Jun in Jurkat and Hela cell lysates using c-Jun rabbit pAb (1:500 diluted).Predicted band size:38kDa.Observed band size:48kDa.)

Western Blot (WB)

(Western Blot analysis of KB cells using AP-1 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of KB cells using AP-1 Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of KB MCF7 cells using AP-1 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of KB MCF7 cells using AP-1 Polyclonal Antibody)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48kDa
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
Jun proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; p39; AP-1; c-Jun
NCBI Protein Information
transcription factor AP-1
UniProt Protein Name
Transcription factor AP-1
Protein Family
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Research Articles on AP-1

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Product Notes

The AP-1 jun (Catalog #AAA477476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AP-1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA). IF: 1:50-200 WB: 1:500-1:2000 IHC: 1:100-1:300 IP: 2-5 ug:mg lysate ELISA: 1:20000. Researchers should empirically determine the suitability of the AP-1 jun for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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