Rabbit anti-Human AOX1 Polyclonal Antibody | anti-AOX1 antibody

AOX1, ID (Aldehyde Oxidase, AO) (FITC)

Cat. Num. AAA6461150

• Gene Names: AOX1; AO; AOH1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: AOX1; Polyclonal Antibody; ID (Aldehyde Oxidase; AO) (FITC); anti-AOX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AOX1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 1338

• Applicable Applications for anti-AOX1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human AOX1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AOX1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). AOX1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(AOX1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-AOX1 antibody

Antibodies; Enzymes; Oxidase

References

1. Wright, R.M., Proc. Natl. Acad. Sci. USA 90(22), 10690-10694 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 438656
• NCBI GeneID: 316
• UniProt Accession #: Q06278
• Molecular Weight: 147.918
• NCBI Official Full Name: aldehyde oxidase
• NCBI Official Synonym Full Names: aldehyde oxidase 1
• NCBI Official Symbol: AOX1
• NCBI Official Synonym Symbols: AO; AOH1
• NCBI Protein Information: aldehyde oxidase
• UniProt Protein Name: Aldehyde oxidase
• Protein Family: Alcohol oxidase
• UniProt Gene Name: AOX1
• UniProt Synonym Gene Names: AO
• UniProt Entry Name: ADO_HUMAN

NCBI Description

Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

AOX1: Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]

Protein type: EC 1.2.3.1; Oxidoreductase; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - vitamin B6

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cytoplasm; cytosol

Molecular Function: molybdopterin cofactor binding; 2 iron, 2 sulfur cluster binding; UDP-N-acetylmuramate dehydrogenase activity; FAD binding; electron carrier activity; iron ion binding; aldehyde oxidase activity; xanthine dehydrogenase activity; NAD binding

Biological Process: vitamin metabolic process; vitamin B6 metabolic process; inflammatory response; water-soluble vitamin metabolic process