Mouse anti-Human ANXA9 Polyclonal Antibody | anti-ANXA9 antibody

ANXA9 (Annexin A9, Annexin XXXI, Annexin-31, Annexin-9, Pemphaxin, ANX31)

Cat. Num. AAA6004046

• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ANXA9; Polyclonal Antibody; ANXA9 (Annexin A9; Annexin XXXI; Annexin-31; Annexin-9; Pemphaxin; ANX31); Anti -ANXA9 (Annexin A9; anti-ANXA9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANXA9.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MAPSLTQEILSHLGLASKTAAWGTLGTLRTFLNFSVDKDAQRLLRAITGQGVDRSAIVDVLTNRSREQRQLISRNFQERTQQDLMKSLQAALSGNLERIVMALLQPTAQFDAQELRTALKASDSAVDVAIEILATRTPPQLQECLAVYKHNFQVEAVDDITSETSGILQDLLLALAKGGRDSYSGIIDYNLAEQDVQALQRAEGPSREETWVPVFTQRNPEHLIRVFDQYQRSTGQELEEAVQNRFHGDAQVALLGLASVIKNTPLYFADKLHQALQETEPNYQVLIRILISRCETDLLSIRAEFRKKFGKSLYSSLQDAVKGDCQSALLALCRAEDM

• Applicable Applications for anti-ANXA9 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human ANXA9, aa1-338 (AAH05830.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ANXA9 polyclonal antibody. Western Blot analysis of ANXA9 expression in human kidney.)

Western Blot (WB)

(Western Blot analysis of ANXA9 expression in transfected 293T cell line by ANXA9 polyclonal antibody. Lane 1: ANXA9 transfected lysate (37.18kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to ANXA9 on HeLa cell. [antibody concentration 10ug/ml].)

Related Product Information for anti-ANXA9 antibody

The annexins are a family of calcium-dependent phospholipid-binding proteins. ANXA9 cDNA encodes a 338aa protein with <40% identity to other annexins. Members of the annexin family contain 4 internal repeat domains, each of which includes a type II calcium-binding site. The calcium-binding sites are required for annexins to aggregate and cooperatively bind anionic phospholipids and extracellular matrix proteins. The most striking feature of annexin 31 is a complete ablation of all four homologous type II calcium-binding sites in the conserved tetrad core. Although all 4 type II calcium-binding sites in annexin 31 contained aa substitutions that ablated their function, structural analysis suggests that the conserved putative ion channel formed by the tetrad core is intact. ANXA9 may act as a low affinity receptor for acetylcholine and is expressed in the stratified squamous skin epithelium, but not in epithelia of other types (at protein level). ANXA9 is one of the target molecules recognized by autoantibodies in patients with pemphigus vulgaris, a rare, autoimmune skin disease in which epidermal blisters occur as the result of the loss of cell-cell adhesion.

Product Categories/Family for anti-ANXA9 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 49168450
• UniProt Accession #: O76027; Q5SZF1; Q6FI55; Q9BS00; Q9HBJ6
• Molecular Weight: 38,364 Da
• NCBI Official Full Name: ANXA9
• UniProt Protein Name: Annexin A9
• Protein Family: Annexin
• UniProt Gene Name: ANXA9
• UniProt Synonym Gene Names: ANX31
• UniProt Entry Name: ANXA9_HUMAN

Uniprot Description

ANXA9: a calcium/phospholipid-binding protein that may act as a low affinity receptor for acetylcholine. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cell surface; cytosol

Molecular Function: protein binding; protein homodimerization activity; calcium-dependent phospholipid binding; phosphatidylserine binding; acetylcholine receptor activity; phospholipid binding; calcium ion binding

Biological Process: synaptic transmission; cell-cell adhesion