Rabbit anti-Human ANXA7 Polyclonal Antibody | anti-ANXA7 antibody

ANXA7, ID (Synexin, Annexin VII, Annexin-7, Annexin A7, ANX7, SNX, OK/SW-cl.95) (Azide free) (HRP)

Cat. Num. AAA6463212

• Gene Names: ANXA7; SNX; ANX7; SYNEXIN
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: ANXA7; Polyclonal Antibody; ID (Synexin; Annexin VII; Annexin-7; Annexin A7; ANX7; SNX; OK/SW-cl.95) (Azide free) (HRP); anti-ANXA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANXA7
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ANXA7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa335-365 in the center region of human ANXA7
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ANXA7 Antibody (Center) in A2058 cell line lysates (35ug/lane). ANXA7 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

((LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with ANXA7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Immunofluorescence analysis of ANXA7 Antibody (Center) with paraffin-embedded human brain tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.)

Flow Cytometry (FC/FACS)

(ANXA7 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ANXA7 antibody

Antibodies; SNX (Sorting Nexin) Proteins

References

1. Shirvan, A., et.al., Biochemistry 33(22), 6888-6901 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 4502111
• NCBI GeneID: 310
• NCBI Accession #: NP_001147.1
• NCBI GenBank Nucleotide #: NM_001156.3
• UniProt Accession #: P20073; Q5F2H3; Q5T0M6; Q5T0M7
• NCBI Official Full Name: annexin A7 isoform 1
• NCBI Official Synonym Full Names: annexin A7
• NCBI Official Symbol: ANXA7
• NCBI Official Synonym Symbols: SNX; ANX7; SYNEXIN
• NCBI Protein Information: annexin A7; annexin VII; annexin-7
• UniProt Protein Name: Annexin A7
• Protein Family: Annexin
• UniProt Gene Name: ANXA7
• UniProt Synonym Gene Names: ANX7; SNX
• UniProt Entry Name: ANXA7_HUMAN

NCBI Description

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA7: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Interacts with PDCD6. Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Belongs to the annexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane; nuclear envelope; cytosol; nucleus

Molecular Function: integrin binding; protein binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell proliferation; cellular water homeostasis; epithelial cell differentiation; hemostasis; autophagy; social behavior; response to calcium ion; response to organic cyclic substance; response to salt stress