Goat ANXA1 / Annexin A1 Polyclonal Antibody | anti-ANXA1 antibody

Goat Polyclonal to Human ANXA1 / Annexin A1

Cat. Num. AAA242449

• Gene Names: ANXA1; ANX1; LPC1
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea Pig, Hamster, Horse, Pig, Rabbit
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ANXA1 / Annexin A1; Polyclonal Antibody; Goat Polyclonal to Human ANXA1 / Annexin A1; Anti-ANXA1 / Annexin A1 Antibody (C-Terminus) IHC-plus; ANXA1; Annexin A1; Annexin I; Annexin-1; ANX1; Calpactin II; Chromobindin-9; Lipocortin I; Annexin I (lipocortin I); Calpactin-2; LPC1; Human ANXA1; anti-ANXA1 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea Pig, Hamster, Horse, Pig, Rabbit
• Clonality: Polyclonal
• Specificity: Human ANXA1 / Annexin A1.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: Recommended Concentration: 0.5-1.5 mg/ml (varies by lot)
• Sequence Length: 346

• Applicable Applications for anti-ANXA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (4 ug/ml), WB (1-3ug/mL), Peptide Enzyme-Linked Immunoabsorbent Assay (1:4000); Optimal dilution should be determined by the researcher.; Usage: Immunohistochemistry: MBS242474 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242474 was determined to be 4 ug/ml.; Peptide ELISA: antibody detection limit dilution 1:3200.; Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7 kD according to NP_000691.1).; The observed molecular weight corresponds to earlier findings in literature with different antibodies ( Paweletz et al, Cancer RES. 2000 Nov15;16(22):6293-7. pmid: 11103786).
• Immunogen Species: Human
• Immunogen: ANXA1 / Annexin A1 antibody was raised against peptide with sequence CQAILDETKGDYEK from the C-terminus of protein sequence according to (NP_000691.1).
• Epitope: aa324-337
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-Annexin A1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Annexin A1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.)

Western Blot (WB)

(Antibody (0.5 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ANXA1 antibody

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35000 to 40000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kD, with phospholipase A2 inhibitory activity.

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NM_000700.2
• UniProt Accession #: P04083
• Molecular Weight: 38,714 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1; annexin-1; calpactin-2; calpactin II; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• Protein Family: Annexin
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1
• UniProt Entry Name: ANXA1_HUMAN

NCBI Description

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

Uniprot Description

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: extracellular space; mast cell granule; protein complex; cell surface; focal adhesion; basolateral plasma membrane; extracellular region; cornified envelope; cilium; mitochondrial membrane; cytoplasm; plasma membrane; nucleus; sarcolemma; vesicle; endosome

Molecular Function: protein binding, bridging; protein binding; single-stranded RNA binding; protein homodimerization activity; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; helicase activity; calcium-dependent protein binding; single-stranded DNA binding; receptor binding

Biological Process: prostate gland development; response to drug; response to peptide hormone stimulus; positive regulation of neutrophil apoptosis; gliogenesis; positive regulation of vesicle fusion; alpha-beta T cell differentiation; arachidonic acid secretion; signal transduction; neutrophil homeostasis; endocrine pancreas development; response to estradiol stimulus; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; cell motility; inflammatory response; negative regulation of apoptosis; positive regulation of prostaglandin biosynthetic process; response to X-ray