Rabbit anti-Human, Mouse Antithrombin III Polyclonal Antibody | anti-ATIII antibody

Antithrombin III, CT (Antithrombin-III, ATIII, PRO0309, AT3, Serpin C1, SERPINC1) (MaxLight 650)

Cat. Num. AAA6463365

• Gene Names: SERPINC1; AT3; AT3D; ATIII; THPH7
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Antithrombin III; Polyclonal Antibody; CT (Antithrombin-III; ATIII; PRO0309; AT3; Serpin C1; SERPINC1) (MaxLight 650); anti-ATIII antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Antithrombin III. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ATIII antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within residues 371-401 in the C-terminal region of human Antithrombin III.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SERPINC1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). SERPINC1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SERPINC1 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SERPINC1 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ATIII antibody

Antibodies; Protease Inhibitors

References

1. Doi, T., Arch. Biochem. Biophys. 489(1-2), 62-67 (2009). 2. Sun, X.J., J. Biol. Chem. 264(19), 11288-11293 (1989).

NCBI and Uniprot Product Information

• NCBI GI #: 4502261
• NCBI GeneID: 462
• NCBI Accession #: NP_000479
• NCBI GenBank Nucleotide #: NM_000488.3
• UniProt Accession #: P01008; P78439; P78447; Q13815; Q5TC78; Q7KZ43; Q7KZ97; Q9UC78; B2R6P0
• Molecular Weight: 51.4kDa (455aa)
• NCBI Official Full Name: antithrombin-III
• NCBI Official Synonym Full Names: serpin family C member 1
• NCBI Official Symbol: SERPINC1
• NCBI Official Synonym Symbols: AT3; AT3D; ATIII; THPH7
• NCBI Protein Information: antithrombin-III
• UniProt Protein Name: Antithrombin-III
• UniProt Gene Name: SERPINC1
• UniProt Synonym Gene Names: AT3; ATIII
• UniProt Entry Name: ANT3_HUMAN

NCBI Description

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]

Uniprot Description

SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protein binding; protease binding

Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient

Disease: Antithrombin Iii Deficiency