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Western Blot (WB) (Western Blot analysis of COLO205 cells using ANT4 Polyclonal Antibody)

Rabbit anti-Human, Mouse ANT4 Polyclonal Antibody | anti-ANT4 antibody

ANT4 Polyclonal Antibody

Gene Names
SLC25A31; AAC4; ANT4; SFEC35kDa
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ANT4; Polyclonal Antibody; ANT4 Polyclonal Antibody; SLC25A31; AAC4; SFEC; ADP/ATP translocase 4; ADP; ATP carrier protein 4; Adenine nucleotide translocator 4; ANT 4; Solute carrier family 25 member 31; Sperm flagellar energy carrier protein; anti-ANT4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
ANT4 Polyclonal Antibody detects endogenous levels of ANT4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
315
Applicable Applications for anti-ANT4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
Immunofluorescence: 1:200 - 1:1000
ELISA: 1:40000
Not yet tested in other applications.
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the Internal region of human ANT4.
Target Name
ANT4
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of COLO205 cells using ANT4 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of COLO205 cells using ANT4 Polyclonal Antibody)
Product Categories/Family for anti-ANT4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,022 Da
NCBI Official Full Name
ADP/ATP translocase 4
NCBI Official Synonym Full Names
solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
NCBI Official Symbol
SLC25A31
NCBI Official Synonym Symbols
AAC4; ANT4; SFEC35kDa
NCBI Protein Information
ADP/ATP translocase 4
UniProt Protein Name
ADP/ATP translocase 4
UniProt Gene Name
SLC25A31
UniProt Synonym Gene Names
AAC4; ANT4; SFEC; ANT 4
UniProt Entry Name
ADT4_HUMAN

NCBI Description

Mitochondrial ADP/ATP carriers, such as SLC25A31, are nuclear-coded mitochondrial proteins that catalyze the exchange of ATP generated in mitochondria by ATP synthase (see MIM 108729) against ADP produced in cytosol by most energy-consuming reactions (Dolce et al., 2005 [PubMed 15670820]).[supplied by OMIM, Mar 2008]

Uniprot Description

SLC25A31: Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May serve to mediate energy generating and energy consuming processes in the distal flagellum, possibly as a nucleotide shuttle between flagellar glycolysis, protein phosphorylation and mechanisms of motility. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Mitochondrial; Transporter

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: transporter activity

Biological Process: transmembrane transport

Research Articles on ANT4

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Product Notes

The ANT4 slc25a31 (Catalog #AAA9404821) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANT4 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ANT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunofluorescence: 1:200 - 1:1000 ELISA: 1:40000 Not yet tested in other applications. Researchers should empirically determine the suitability of the ANT4 slc25a31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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