Rabbit anti-Human ANO6 Polyclonal Antibody | anti-ANO6 antibody

ANO6, NT (ANO6, TMEM16F, Anoctamin-6, Transmembrane protein 16F) (MaxLight 750)

Cat. Num. AAA6273816

• Gene Names: ANO6; SCTS; BDPLT7; TMEM16F
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANO6; Polyclonal Antibody; NT (ANO6; TMEM16F; Anoctamin-6; Transmembrane protein 16F) (MaxLight 750); Transmembrane protein 16F; anti-ANO6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANO6.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-ANO6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 68-96 in the N-terminal region of human ANO6, UniProt Accession #Q4KMQ2.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in SW480 cell line lysates (35ug/lane) usingMBS6004053. This demonstrates that MBS6004053 detected the ANO6 protein (arrow).)

Related Product Information for anti-ANO6 antibody

MaxLight750 is a new Near IR stable dye conjugate comparable to DyLight750, Alexa Fluor700 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.

Product Categories/Family for anti-ANO6 antibody

Antibodies; Transmembrane Proteins

References

1. Rose, J. Phd, et al. Mol. Med. (2010). In press 2. Hartzell, H.C., et al. J. Physiol. (Lond.) 587 (PT 10), 2127-2139 (2009). 3. Katoh, M., et al. Int. J. Oncol. 24(5):1345-1349(2004). 4. Adams, M.D., et al. Nature 377 (6547 SUPPL), 3-174 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 218156299
• NCBI GeneID: 196527
• NCBI Accession #: NP_001020527.2
• NCBI GenBank Nucleotide #: NM_001025356.2
• UniProt Accession #: Q4KMQ2; Q8N3Q2; A6NNM6; B9EGG0; E7ENK4; E9PB30; E9PCT2
• Molecular Weight: 108,102 Da
• NCBI Official Full Name: anoctamin-6 isoform a
• NCBI Official Synonym Full Names: anoctamin 6
• NCBI Official Symbol: ANO6
• NCBI Official Synonym Symbols: SCTS; BDPLT7; TMEM16F
• NCBI Protein Information: anoctamin-6
• UniProt Protein Name: Anoctamin-6
• Protein Family: Anoctamin
• UniProt Gene Name: ANO6
• UniProt Synonym Gene Names: TMEM16F; SCAN channel

NCBI Description

This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (). Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.