Western Blot (WB)
(Biotinylated (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat Annexin A11 Polyclonal Antibody | anti-ANXA11 antibody
Goat anti-Annexin A11, biotinylated Antibody
Western Blot: Approx 55kDa band observed in Jurkat lysates (calculated MW of 54.4kDa according to NP_001148.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
(Biotinylated (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. [provided by RefSeq, Dec 2015]
Uniprot Description
ANXA11: a calcium/phospholipid-binding protein found throughout the nucleoplasm at interphase and at the mitotic phase is concentrated at the loop-like structure around the mitotic apparatus. May interact with calcyclin (S100A6) in vivo. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
Protein type: Nuclear envelope; Lipid-binding; Calcium-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q23
Cellular Component: azurophil granule; cytoplasm; melanosome; membrane; midbody; nuclear envelope; nucleoplasm; phagocytic vesicle; specific granule; spindle
Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; phosphatidylethanolamine binding; protein binding
Biological Process: cell cycle; cell division; phagocytosis; response to calcium ion
Research Articles on ANXA11
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Product Notes
The ANXA11 anxa11 (Catalog #AAA423589) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Annexin A11, biotinylated Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A11 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 55kDa band observed in Jurkat lysates (calculated MW of 54.4kDa according to NP_001148.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ANXA11 anxa11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: YPGYPPPPGG YPP. It is sometimes possible for the material contained within the vial of "Annexin A11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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