Rabbit anti-Human, Mouse ANKRD27 Polyclonal Antibody | anti-ANKRD27 antibody

ANKRD27 Antibody

Cat. Num. AAA9630299

• Gene Names: ANKRD27; VARP; PP12899
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ANKRD27; Polyclonal Antibody; ANKRD27 Antibody; AA408090; Ankrd27; Ankyrin repeat domain 27 (VPS9 domain); Ankyrin repeat domain-containing protein 27; ANR27_HUMAN; BC016493; D330003H11Rik; DKFZp434L0718; FLJ00040; MGC25907; PP12899; VPS9 ankyrin repeat protein; VPS9 domain-containing protein; anti-ANKRD27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ANKRD27 Antibody detects endogenous levels of ANKRD27.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ANKRD27 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ANKRD27.
• Conjugation: Unconjugated
• Subunit Structure: Interacts with RAB21 (GDP-bound form), VPS29, RAB32 (GTP-bound form), RAB38 (GTP-bound form), VAMP7, KIF5A, KIF5C, GOLGA4. Interacts with low affinity with RAB5. ANKRD27:RAB32 heterodimers can homodimerize to form tetramers. Can interact with RAB38 or RAB32, VPS29 and VAMP7 simultaneously. A decreased interaction with RAB32 seen in the presence of SGSM2.
• Function: May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics. May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation. Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites. Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29. Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).
• Note: Colocalizes with VAMP7 in transport vesicles in the shaft of hippocampal neurons (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ANKRD27 antibody

Early endosome; Late endosome; Cytoplasmic vesicle membrane; Lysosome; Cell membrane; Melanosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 116063534
• NCBI GeneID: 84079
• NCBI Accession #: NP_115515
• NCBI GenBank Nucleotide #: NM_032139.2
• UniProt Accession #: Q96NW4; Q71MF5; Q86UC3; Q8ND80; Q9H0I4
• Molecular Weight: 116,984 Da
• NCBI Official Full Name: ankyrin repeat domain-containing protein 27
• NCBI Official Synonym Full Names: ankyrin repeat domain 27 (VPS9 domain)
• NCBI Official Symbol: ANKRD27
• NCBI Official Synonym Symbols: VARP; PP12899
• NCBI Protein Information: ankyrin repeat domain-containing protein 27; VPS9-ankyrin-repeat protein; VPS9 domain-containing protein; Vps9 domain and ankyrin-repeat-containing protein
• UniProt Protein Name: Ankyrin repeat domain-containing protein 27
• Protein Family: Ankyrin repeat domain-containing protein
• UniProt Gene Name: ANKRD27
• UniProt Entry Name: ANR27_HUMAN

Uniprot Description

ANKRD27: May be a Rab21 guanine exchange factor and regulate endosome dynamics.

Protein type: GEFs; GEFs, Rab

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: cytoplasmic vesicle membrane; membrane; lysosome; cytoplasm; late endosome; early endosome; plasma membrane; melanosome

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; Rab GTPase binding; GTPase activator activity

Biological Process: protein transport; early endosome to late endosome transport; positive regulation of GTPase activity