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Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 60 μ g Lane 1-3: Mouse lung tissue, NIH/3T3 cells, hepg2 cells Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds)

Rabbit anti-Human, Mouse ANKRD1 Polyclonal Antibody | anti-ANKRD1 antibody

ANKRD1 Antibody

Gene Names
ANKRD1; ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
ANKRD1; Polyclonal Antibody; ANKRD1 Antibody; ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2; anti-ANKRD1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ANKRD1 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.6 mg/ml (varies by lot)
Sequence Length
319
Applicable Applications for anti-ANKRD1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Immunogen
Fusion protein of human ANKRD1
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 8%SDS-PAGE Lysate: 60 μ g Lane 1-3: Mouse lung tissue, NIH/3T3 cells, hepg2 cells Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds)

Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 60 μ g Lane 1-3: Mouse lung tissue, NIH/3T3 cells, hepg2 cells Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using at dilution 1/20.)
Related Product Information for anti-ANKRD1 antibody
The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor.Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system.
Product Categories/Family for anti-ANKRD1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,252 Da
NCBI Official Full Name
ankyrin repeat domain-containing protein 1
NCBI Official Synonym Full Names
ankyrin repeat domain 1
NCBI Official Symbol
ANKRD1
NCBI Official Synonym Symbols
ALRP; CARP; C-193; CVARP; MCARP; bA320F15.2
NCBI Protein Information
ankyrin repeat domain-containing protein 1
UniProt Protein Name
Ankyrin repeat domain-containing protein 1
UniProt Gene Name
ANKRD1
UniProt Synonym Gene Names
C193; CARP; HA1A2
UniProt Entry Name
ANKR1_HUMAN

Uniprot Description

ANKRD1: May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells. Defects in ANKRD1 may be a cause of total anomalous pulmonary venous return (TAPVR). TAPVR is a rare congenital heart disease (CHD) in which the pulmonary veins fail to connect to the left atrium during cardiac development, draining instead into either the right atrium or one of its venous tributaries. This disease accounts for 1.5% of all CHDs and has a prevalence of approximately 1 out of 15'000 live births.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: cytoplasm; cytosol; I band; nucleolus; nucleoplasm; nucleus; sarcomere

Molecular Function: DNA binding; histone deacetylase binding; p53 binding; protein binding; titin binding; transcription corepressor activity

Biological Process: cardiac muscle morphogensis; muscle cell differentiation; positive regulation of apoptosis; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of protein secretion

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Product Notes

The ANKRD1 ankrd1 (Catalog #AAA9416276) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANKRD1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ANKRD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the ANKRD1 ankrd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANKRD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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