Rabbit ANKLE2 Polyclonal Antibody | anti-ANKLE2 antibody

ANKLE2 Polyclonal Antibody

Cat. Num. AAA3005816

• Gene Names: ANKLE2; Lem4; LEMD7; KIAA0692
• Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep
• Applications: Western Blot
• Purity: affinity purified by Protein A
• Synonyms: ANKLE2; Polyclonal Antibody; ANKLE2 Polyclonal Antibody; ANKL2_HUMAN; ANKLE 2; ankyrin repeat and LEM domain containing 2; Ankyrin repeat and LEM domain-containing protein 2; LEM domain containing 7; LEMD 7; LEMD7; anti-ANKLE2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep
• Clonality: Polyclonal
• Specificity: ANKLE2 Polyclonal Antibody detects endogenous levels of ANKLE2 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-ANKLE2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-2000
• Immunogen: KLH conjugated synthetic peptide derived from human ANKLE2:251-350/938
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Primary: Anti-ANKLE2 at 1/1000 dilution)

Related Product Information for anti-ANKLE2 antibody

Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKLE2 (ankyrin repeat and LEM domain containing 2), also known as LEMD7, is a 938 amino acid single-pass membrane protein containing an ANK repeat and a LEM domain. Exsiting as two isoforms produced by alternative splicing events, the gene encoding ANKLE2 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders.

NCBI and Uniprot Product Information

• NCBI GI #: 148664230
• NCBI GeneID: 23141
• NCBI Accession #: AAH43157
• NCBI GenBank Nucleotide #: NM_015114.2
• UniProt Accession #: Q86XL3; O75176; Q6P6A5; Q8TAZ9; Q96DH4; A8KAG3; B3KN97; B3KSF8
• Molecular Weight: 31,982 Da
• NCBI Official Full Name: ankyrin repeat and LEM domain-containing protein 2
• NCBI Official Synonym Full Names: ankyrin repeat and LEM domain containing 2
• NCBI Official Symbol: ANKLE2
• NCBI Official Synonym Symbols: Lem4; LEMD7; KIAA0692
• NCBI Protein Information: ankyrin repeat and LEM domain-containing protein 2; LEM domain containing 7; LEM domain-containing protein 4
• UniProt Protein Name: Ankyrin repeat and LEM domain-containing protein 2
• Protein Family: Ankyrin repeat and LEM domain-containing protein
• UniProt Gene Name: ANKLE2
• UniProt Synonym Gene Names: KIAA0692; LEM4
• UniProt Entry Name: ANKL2_HUMAN

NCBI Description

This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014]

Uniprot Description

ANKLE2: Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Belongs to the ANKLE2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: endoplasmic reticulum membrane; membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; protein phosphatase 2A binding; protein phosphatase type 2A regulator activity

Biological Process: mitosis; mitotic nuclear envelope reassembly; cell division; regulation of catalytic activity; negative regulation of phosphorylation; mitotic cell cycle; positive regulation of protein amino acid dephosphorylation