Rabbit anti-Human, Mouse ANGPTL4 Polyclonal Antibody | anti-ANGPTL4 antibody

ANGPTL4, ID (ANGPTL4, ARP4, HFARP, PGAR, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein)

Cat. Num. AAA6004494

• Gene Names: ANGPTL4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; UNQ171; pp1158; ANGPTL2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ANGPTL4; Polyclonal Antibody; ID (ANGPTL4; ARP4; HFARP; PGAR; Angiopoietin-related protein 4; Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein); Anti -ANGPTL4; anti-ANGPTL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ANGPTL4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: ANGPTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145~175 amino acids from the Center region of human ANGPTL4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ANGPTL4 Antibody (Center) in mouse liver tissue lysates (35ug/lane). ANGPTL4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ANGPTL4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ANGPTL4 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ANGPTL4 antibody

ANGPTL4 is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This protein is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes.

Product Categories/Family for anti-ANGPTL4 antibody

Antibodies; Abs to Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 89264696
• NCBI GeneID: 51129
• NCBI Accession #: NP_001034756.1
• NCBI GenBank Nucleotide #: NM_001039667.2
• UniProt Accession #: Q9BY76; Q53HQ6; Q53HU1; Q6UXN0; Q9HBV4; Q9NZU4; Q9Y5B3; A8MY84; D6W670
• Molecular Weight: 45,214 Da
• NCBI Official Full Name: angiopoietin-related protein 4 isoform b
• NCBI Official Synonym Full Names: angiopoietin-like 4
• NCBI Official Symbol: ANGPTL4
• NCBI Official Synonym Symbols: NL2; ARP4; FIAF; HARP; PGAR; HFARP; UNQ171; pp1158; ANGPTL2
• NCBI Protein Information: angiopoietin-related protein 4; fasting-induced adipose factor; PPARG angiopoietin related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein
• UniProt Protein Name: Angiopoietin-related protein 4
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL4
• UniProt Synonym Gene Names: ARP4; HFARP; PGAR; HFARP
• UniProt Entry Name: ANGL4_HUMAN

NCBI Description

This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013]

Uniprot Description

ANGPTL4: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

Protein type: Apoptosis; Secreted, signal peptide; Extracellular matrix; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: enzyme inhibitor activity; protein binding

Biological Process: positive regulation of angiogenesis; response to hypoxia; negative regulation of lipoprotein lipase activity; angiogenesis; cellular lipid metabolic process; cell differentiation; protein homooligomerization; negative regulation of apoptosis

Disease: Plasma Triglyceride Level Quantitative Trait Locus