Rabbit anti-Human Angiotensin I Converting Enzyme Polyclonal Antibody | anti-ACE antibody

Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE)

Cat. Num. AAA2028654

• Gene Names: ACE; DCP; ACE1; DCP1; CD143
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Angiotensin I Converting Enzyme; Polyclonal Antibody; Polyclonal Antibody to Angiotensin I Converting Enzyme (ACE); anti-ACE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)

• Applicable Applications for anti-ACE antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Organism Species: Homo sapiens (Human)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Human
• Immunogen: Recombinant ACE (Ser1160~Ser1306) expressed in E.coli (MBS2029765)
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant ACE, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Skin Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Stomach Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human ACE AntibodySecond Ab: 2ug/mL HRP-LinkedCaprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Breast cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human ACE AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

• NCBI GI #: 4503273
• NCBI GeneID: 1636
• NCBI Accession #: NP_000780.1
• NCBI GenBank Nucleotide #: NM_000789.3
• UniProt Accession #: P12821; P22966; Q53YX9; Q59GY8; Q7M4L4; B0LPF0; B4DXI3; E7EU16
• NCBI Official Full Name: angiotensin-converting enzyme isoform 1
• NCBI Official Synonym Full Names: angiotensin I converting enzyme
• NCBI Official Symbol: ACE
• NCBI Official Synonym Symbols: DCP; ACE1; DCP1; CD143
• NCBI Protein Information: angiotensin-converting enzyme
• UniProt Protein Name: Angiotensin-converting enzyme
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: ACE
• UniProt Synonym Gene Names: DCP; DCP1; ACE

NCBI Description

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]

Uniprot Description

ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.15.1; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: endosome; external side of plasma membrane; extracellular exosome; extracellular region; extracellular space; integral component of membrane; lysosome; plasma membrane

Molecular Function: bradykinin receptor binding; carboxypeptidase activity; chloride ion binding; drug binding; endopeptidase activity; exopeptidase activity; metallopeptidase activity; mitogen-activated protein kinase binding; mitogen-activated protein kinase kinase binding; peptidyl-dipeptidase activity; tripeptidyl-peptidase activity; zinc ion binding

Biological Process: angiotensin catabolic process in blood; angiotensin maturation; antigen processing and presentation of peptide antigen via MHC class I; arachidonic acid secretion; beta-amyloid metabolic process; blood vessel remodeling; cell proliferation in bone marrow; heart contraction; hormone catabolic process; kidney development; mononuclear cell proliferation; neutrophil mediated immunity; peptide catabolic process; positive regulation of peptidyl-cysteine S-nitrosylation; positive regulation of peptidyl-tyrosine autophosphorylation; proteolysis; regulation of angiotensin metabolic process; regulation of blood pressure; regulation of blood vessel diameter; regulation of hematopoietic stem cell proliferation; regulation of renal output by angiotensin; regulation of smooth muscle cell migration; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of vasoconstriction; spermatogenesis

Disease: Alzheimer Disease; Hemorrhage, Intracerebral, Susceptibility To; Microvascular Complications Of Diabetes, Susceptibility To, 3; Renal Tubular Dysgenesis