Western Blot (WB)
(Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.)
Rabbit anti-Human AMT Polyclonal Antibody | anti-AMT antibody
AMT, NT (Aminomethyltransferase, Mitochondrial, Glycine Cleavage System T Protein, GCVT, GCST) (APC)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(AMT Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(AMT Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
AMT: The glycine cleavage system catalyzes the degradation of glycine. Defects in AMT are a cause of non-ketotic hyperglycinemia (NKH); also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. Belongs to the GcvT family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - glycine, serine and threonine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.10; Energy Metabolism - nitrogen; Methyltransferase; Mitochondrial
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: mitochondrial matrix; mitochondrion
Molecular Function: aminomethyltransferase activity
Biological Process: glycine catabolic process; glycine decarboxylation via glycine cleavage system; glyoxylate metabolic process
Disease: Glycine Encephalopathy
Research Articles on AMT
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Product Notes
The AMT amt (Catalog #AAA6462828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMT, NT (Aminomethyltransferase, Mitochondrial, Glycine Cleavage System T Protein, GCVT, GCST) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AMT amt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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