Immunohistochemistry (IHC)
(Anti-NIF3L1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Goat ALS2CR1 / NIF3L1 Polyclonal Antibody | anti-ALS2CR1 antibody
Goat Polyclonal to Human ALS2CR1 / NIF3L1
Gene Names
NIF3L1; CALS-7; MDS015; ALS2CR1
Reactivity
Gibbon, Gorilla, Human, MonkeyPredicted Reactivity: Mouse, Rat, Bat, Dog, Hamster, Horse, Chicken (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
ALS2CR1 / NIF3L1; Polyclonal Antibody; Goat Polyclonal to Human ALS2CR1 / NIF3L1; Anti-ALS2CR1 / NIF3L1 Antibody (C-Terminus) IHC-plus; NIF3L1; CALS-7; MDS015; NIF3-like protein 1; ALS2CR1; Human ALS2CR1; anti-ALS2CR1 antibody
Host
Goat
Reactivity
Gibbon, Gorilla, Human, Monkey
Predicted Reactivity: Mouse, Rat, Bat, Dog, Hamster, Horse, Chicken (at least 90% immunogen sequence identity)
Predicted Reactivity: Mouse, Rat, Bat, Dog, Hamster, Horse, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human NIF3L1. This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1; NP_068596.2) encode the same isoform.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
350
Applicable Applications for anti-ALS2CR1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:32000), IHC-P (2.5 ug/ml), WB (0.2 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
Synthetic peptide C-ILSETDRDPLQVV from the C-terminus of human NIF3L1 (NP_001129511.1; NP_068596.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%).
Immunogen Type
Synthetic peptide
Immunogen
ALS2CR1 / NIF3L1 antibody was raised against synthetic peptide C-ILSETDRDPLQVV from the C-terminus of human NIF3L1 (NP_001129511.1; NP_068596.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%); Mouse, Rat, Hamster, Panda, Bat, Dog, Horse, Turkey, Chicken (92%); Goat, Elephant, Bovine (85%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Anti-NIF3L1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-NIF3L1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Antibody staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)
Western Blot (WB)
(Antibody staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,164 Da
NCBI Official Full Name
putative GTP cyclohydrolase 1 type 2 NIF3L1 isoform 2
NCBI Official Synonym Full Names
NIF3 NGG1 interacting factor 3-like 1 (S. cerevisiae)
NCBI Official Symbol
NIF3L1
NCBI Official Synonym Symbols
CALS-7; MDS015; ALS2CR1
NCBI Protein Information
putative GTP cyclohydrolase 1 type 2 NIF3L1; NIF3-like protein 1; GTP cyclohydrolase I; NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 1 protein
UniProt Protein Name
Putative GTP cyclohydrolase 1 type 2 NIF3L1
UniProt Gene Name
NIF3L1
UniProt Synonym Gene Names
ALS2CR1
UniProt Entry Name
GTPC1_HUMAN
Uniprot Description
NIF3L1: Belongs to the UPF0135 (NIF3) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.4.16; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: mitochondrion; cytoplasm
Molecular Function: GTP cyclohydrolase I activity; protein binding; GTP binding; metal ion binding; transcription factor binding
Biological Process: positive regulation of transcription, DNA-dependent
Research Articles on ALS2CR1
Similar Products
Product Notes
The ALS2CR1 nif3l1 (Catalog #AAA242199) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human ALS2CR1 / NIF3L1 reacts with Gibbon, Gorilla, Human, Monkey Predicted Reactivity: Mouse, Rat, Bat, Dog, Hamster, Horse, Chicken (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's ALS2CR1 / NIF3L1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:32000), IHC-P (2.5 ug/ml), WB (0.2 - 1 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the ALS2CR1 nif3l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALS2CR1 / NIF3L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
