Western Blot (WB)
(Western Blot (WB) analysis of ALPK1 polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:HCC827 whole cell lysate (40ug))
Rabbit anti-Human ALPK1 Polyclonal Antibody | anti-ALPK1 antibody
ALPK1 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
ALPK1; Polyclonal Antibody; ALPK1 Polyclonal Antibody; Alpha-protein kinase 1; Chromosome 4 kinase; Lymphocyte alpha-protein kinase; anti-ALPK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ALPK1 polyclonal antibody detects endogenous levels of ALPK1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1244
Applicable Applications for anti-ALPK1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 11-55 of Human ALPK1
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ALPK1 polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:HCC827 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of ALPK1 polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:HCC827 whole cell lysate (40ug))
Related Product Information for anti-ALPK1 antibody
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. alpha-protein kinase 1, also known as ALPK1, LAK or KIAA1527, is a 1, 244 amino acid protein that contains one alpha-type protein kinase domain and belongs to the protein kinase superfamily. Expressed at high levels in liver, alpha-protein kinase 1 recognizes and phosphorylates specific phosphorylation sites that are surrounded by peptides which have an å-helical conformation. One of the targets of å-protein kinase 1 is Myosin I and, via its ability to phosphorylate Myosin I, alpha-protein kinase 1 is thought to play an important role in the apical trafficking of vesicles carrying raft-associated sucrase-isomaltase (SI).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 139kDa
NCBI Official Full Name
alpha-protein kinase 1 isoform 1
UniProt Protein Name
Alpha-protein kinase 1
UniProt Gene Name
ALPK1
UniProt Synonym Gene Names
KIAA1527; LAK
UniProt Entry Name
ALPK1_HUMAN
Uniprot Description
AlphaK3: Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
Protein type: Kinase, protein; EC 2.7.11.-; Protein kinase, atypical; ATYPICAL group; Alpha family
Chromosomal Location of Human Ortholog: 4q25
Molecular Function: protein serine/threonine kinase activity; ATP binding
Biological Process: protein amino acid phosphorylation
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Product Notes
The ALPK1 alpk1 (Catalog #AAA3007454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALPK1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the ALPK1 alpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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