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Western Blot (WB) (Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa.)

Rabbit anti-Human alpha Tubulin Polyclonal Antibody | anti-TUBA1B antibody

alpha Tubulin Antibody: RPE

Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Peptide Affinity Purified
Synonyms
alpha Tubulin; Polyclonal Antibody; alpha Tubulin Antibody: RPE; Rabbit Anti-Human alpha Tubulin Antibody Polyclonal; Alpha-tubulin ubiquitous Antibody; Alpha tubulin ubiquitous Antibody; Tubulin alpha 1B chain Antibody; Tubulin alpha ubiquitous Antibody; Tubulin K-alpha-1 Antibody; TBA1B_HUMAN Antibody; K alpha 1 Antibody; Tubulin alpha ubiquitous chain Antibody; Tubulin alpha 1B Antibody; TUBA1B Antibody; Tubulin alpha-1B chain Antibody; Tubulin alpha-ubiquitous chain Antibody; Tubulin K alpha 1 Antibody; anti-TUBA1B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Detects ~50 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
451
Applicable Applications for anti-TUBA1B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000
ICC/IF: 1:100
Target
alpha Tubulin Antibody
Immunogen
Synthetic peptide of Human alpha-Tubulin
Immunogen Species
Human
Cellular Localization
Cytoplasm; Cytoskeleton
Conjugate
RPE
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa.)

Western Blot (WB) (Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa.)

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa.)

Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,218 Da
NCBI Official Full Name
tubulin alpha-1B chain
NCBI Official Synonym Full Names
tubulin alpha 1b
NCBI Official Symbol
TUBA1B
NCBI Official Synonym Symbols
K-ALPHA-1
NCBI Protein Information
tubulin alpha-1B chain
UniProt Protein Name
Tubulin alpha-1B chain
UniProt Gene Name
TUBA1B

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Research Articles on TUBA1B

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Product Notes

The TUBA1B tuba1b (Catalog #AAA8000155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha Tubulin Antibody: RPE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ICC/IF: 1:100. Researchers should empirically determine the suitability of the TUBA1B tuba1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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