Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human testis using MBS766566 (tubulin-Alpha antibody) at dilution of 1:50)
Rabbit alpha Tubulin Polyclonal Antibody
alpha 1a Tubulin Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat; other species are not tested.
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Immunogen affinity purified>=95% as determined by SDS-PAGE
Synonyms
alpha Tubulin; Polyclonal Antibody; alpha 1a Tubulin Rabbit Polyclonal Antibody; Alpha tubulin; TUBA1A; TUBA3; anti-alpha Tubulin antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat; other species are not tested.
Clonality
Polyclonal
Isotype
IgG
Specificity
Please decide specifity by homology.
Purity/Purification
Immunogen affinity purified
>=95% as determined by SDS-PAGE
>=95% as determined by SDS-PAGE
Form/Format
Liquid
Applicable Applications for anti-alpha Tubulin antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB : 1:500-1:5000
IF: 1:10-1:100
IHC: 1:20-1:200
IF: 1:10-1:100
IHC: 1:20-1:200
Immunogen
Tubulin, alpha 1a
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Calculated MW
50 kDa
Preparation and Storage
Store at -20°C for 24 months (Avoid repeated freeze/thaw cycles.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human testis using MBS766566 (tubulin-Alpha antibody) at dilution of 1:50)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human testis using MBS766566 (tubulin-Alpha antibody) at dilution of 1:50)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS766566 (tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS766566 (tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG)
Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS766566 (tubulin-Alpha antibody) at dilution of 1:1000)
Western Blot (WB)
(HeLa cells were subjected to SDS PAGE followed by western blot with MBS766566 (tubulin-Alpha antibody) at dilution of 1:1000)
Related Product Information for anti-alpha Tubulin antibody
Alpha la Tubulin is also known as Alpha-tubulin 3. Tubulin is the major constituent of micro tubules. It binds two moles of GTP, one at an exchangeable site on the beta cha5n and one at a non-exchangeable site on the alpha chain. Alpha 1 a Tubulin is expressed at a high level in fetal brain.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
50kDa
Uniprot Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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Product Notes
The alpha Tubulin (Catalog #AAA766566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha 1a Tubulin Rabbit Polyclonal Antibody reacts with Human, Mouse, Rat; other species are not tested. and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Tubulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB : 1:500-1:5000 IF: 1:10-1:100 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the alpha Tubulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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