Western Blot (WB)
(Western blot analysis of Alpha Defensin 1 expression in rat testis extract (lane 1) and HELA whole cell lysates (lane 2). Alpha Defensin 1 at 19KD was detected using rabbit anti- Alpha Defensin 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit anti-Human, Rat Alpha Defensin 1 Polyclonal Antibody | anti-DEFA1 antibody
Anti-Alpha Defensin 1 Antibody
Each vial contains 4mg Trehalouse, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be deteremined by end users.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of Alpha Defensin 1 expression in rat testis extract (lane 1) and HELA whole cell lysates (lane 2). Alpha Defensin 1 at 19KD was detected using rabbit anti- Alpha Defensin 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of Alpha Defensin 1 expression in rat testis extract (lane 1) and HELA whole cell lysates (lane 2). Alpha Defensin 1 at 19KD was detected using rabbit anti- Alpha Defensin 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Background: Defensin, alpha 1, also known as human alpha defensin 1, human neutrophil peptide 1 (HNP-1) or neutrophil defensin 1 is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.
2. Aldred PM, Hollox EJ, Armour JA (Jul 2005). "Copy number polymorphism and expression level variation of the human alpha-defensin genes DEFA1 and DEFA3".Hum Mol Genet. 14 (14): 2045-52.
3. Feng Y, Gutekunst CA, Eberhart DE, Yi H, Warren ST, Hersch SM (Mar 1997). "Fragile X mental retardation protein: nucleocytoplasmic shuttling and association with somatodendritic ribosomes". J Neurosci. 17 (5): 1539-47.
NCBI and Uniprot Product Information
NCBI Description
Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
Uniprot Description
defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: extracellular matrix; extracellular region; extracellular space; Golgi lumen
Biological Process: antibacterial humoral response; chemotaxis; defense response to Gram-positive bacterium; estrogen receptor signaling pathway; immune response; innate immune response; innate immune response in mucosa
Research Articles on DEFA1
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Product Notes
The DEFA1 defa1 (Catalog #AAA178711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha Defensin 1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha Defensin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu, Rat Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be deteremined by end users. Researchers should empirically determine the suitability of the DEFA1 defa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Defensin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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