Rabbit alpha B Crystallin Polyclonal Antibody | anti-CRYAB antibody

Alpha B Crystallin Antibody: HRP

Cat. Num. AAA805343

• Gene Names: CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• Reactivity: Human, Mouse, Rat, Cow, Chicken
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Synonyms: alpha B Crystallin; Polyclonal Antibody; Alpha B Crystallin Antibody: HRP; AACRYA; Alpha crystallin B chain; CRYA2; CRYAB; CTPP2; HspB5; NY REN 27 antigen; anti-CRYAB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Cow, Chicken
• Clonality: Polyclonal
• Form/Format: Rabbit Antiserum
• Sequence Length: 175

• Applicable Applications for anti-CRYAB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: 1:5000-10000 (ECL) (WB)
• Conjugate: HRP
• Storage Buffer: Rabbit antiserum
• Preparation and Storage: -20 degree C

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Western Blot (WB)

(Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Western Blot (WB)

(Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.)

Related Product Information for anti-CRYAB antibody

Background Info: Detects ~22kDa. Does not cross-react with alphaA-crystallin.

Scientific Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).

Product Categories/Family for anti-CRYAB antibody

Chaperones; Heat Shock

References

1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203- 215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577. 9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.

NCBI and Uniprot Product Information

• NCBI GI #: 4503057
• NCBI GeneID: 1410
• NCBI Accession #: NP_001876.1
• NCBI GenBank Nucleotide #: NM_001885.2
• UniProt Accession #: P02511; O43416; Q9UC37; Q9UC38; Q9UC39; Q9UC40; Q9UC41; B0YIX0
• Molecular Weight: 20,159 Da
• NCBI Official Full Name: alpha-crystallin B chain
• NCBI Official Synonym Full Names: crystallin, alpha B
• NCBI Official Symbol: CRYAB
• NCBI Official Synonym Symbols: MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• NCBI Protein Information: alpha-crystallin B chain; heat shock protein beta-5; rosenthal fiber component; heat-shock 20 kD like-protein; renal carcinoma antigen NY-REN-27; epididymis secretory protein Li 101
• UniProt Protein Name: Alpha-crystallin B chain
• Protein Family: Alpha-crystallin
• UniProt Gene Name: CRYAB
• UniProt Synonym Gene Names: CRYA2; HspB5
• UniProt Entry Name: CRYAB_HUMAN

NCBI Description

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 11q22.3-q23.1

Cellular Component: microtubule cytoskeleton; Golgi apparatus; cell surface; mitochondrion; cytoplasm; plasma membrane; actin filament bundle; cytosol; nucleus; Z disc

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; microtubule binding; unfolded protein binding; structural constituent of eye lens

Biological Process: lens development in camera-type eye; muscle development; protein folding; microtubule polymerization or depolymerization; stress-activated MAPK cascade; negative regulation of caspase activity; response to estradiol stimulus; tubulin folding; muscle contraction; negative regulation of intracellular transport; response to hydrogen peroxide; response to hypoxia; negative regulation of cell growth; protein homooligomerization; negative regulation of apoptosis; aging

Disease: Cataract 16, Multiple Types; Myopathy, Myofibrillar, Fatal Infantile Hypertonic, Alpha-b Crystallin-related; Myopathy, Myofibrillar, 2; Cardiomyopathy, Dilated, 1ii