Western Blot (WB)
(Western Blot: Sample: Mouse Serum; Primary Ab: 2ug/ml Rabbit Anti-Mouse a1AGP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Alpha-1-Acid Glycoprotein (a1AGP) Polyclonal Antibody | anti-a1AGP antibody
Polyclonal Antibody to Alpha-1-Acid Glycoprotein (a1AGP)
Gene Names
Orm1; Agp-1; Agp-2; Orm-1
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Alpha-1-Acid Glycoprotein (a1AGP); Polyclonal Antibody; Polyclonal Antibody to Alpha-1-Acid Glycoprotein (a1AGP); anti-a1AGP antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against a1AGP. It has been selected for its ability to recognize a1AGP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-QN PEHANFTIGE PITNETLSWL SDKWFFMGAA FRKLEYRQAI QTMQSEFFYL TTNLINDTIE LRESQTIGDQ CVYNSTHLGF QRENGTFSKY EGGVETFAHL IVLRKHGAFM LAFDLKDEKK RGLSLYAKRP DITPELREVF QKAVTHVGMD ESEIIFVDWK KDRCGQQEKK QLELGKETKK DPEEGQA
MGHHHHHHSGSEF-QN PEHANFTIGE PITNETLSWL SDKWFFMGAA FRKLEYRQAI QTMQSEFFYL TTNLINDTIE LRESQTIGDQ CVYNSTHLGF QRENGTFSKY EGGVETFAHL IVLRKHGAFM LAFDLKDEKK RGLSLYAKRP DITPELREVF QKAVTHVGMD ESEIIFVDWK KDRCGQQEKK QLELGKETKK DPEEGQA
Sequence Length
201
Applicable Applications for anti-a1AGP antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant a1AGP (Gln19~Ala207) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047898
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Serum; Primary Ab: 2ug/ml Rabbit Anti-Mouse a1AGP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Serum; Primary Ab: 2ug/ml Rabbit Anti-Mouse a1AGP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,895 Da
NCBI Official Full Name
alpha-1-acid glycoprotein 1
NCBI Official Synonym Full Names
orosomucoid 1
NCBI Official Symbol
Orm1
NCBI Official Synonym Symbols
Agp-1; Agp-2; Orm-1
NCBI Protein Information
alpha-1-acid glycoprotein 1
UniProt Protein Name
Alpha-1-acid glycoprotein 1
UniProt Gene Name
Orm1
UniProt Synonym Gene Names
Agp1; Orm-1; AGP 1; OMD 1
NCBI Description
This gene encodes a member of the lipocalin family of proteins. The encoded protein is an abundant acute-phase protein that is synthesized by hepatocytes in response to cytokines during the acute phase response. The encoded protein may regulate inflammation and metabolism. This gene is present in a gene cluster on chromosome 4. [provided by RefSeq, Aug 2015]
Uniprot Description
ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4 B3|4 33.96 cM
Cellular Component: extracellular region; extracellular space
Biological Process: acute-phase response; regulation of immune system process; transport
Research Articles on a1AGP
Similar Products
Product Notes
The a1AGP orm1 (Catalog #AAA2003607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alpha-1-Acid Glycoprotein (a1AGP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-1-Acid Glycoprotein (a1AGP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the a1AGP orm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-QN PEHANFTIGE PITNETLSWL SDKWFFMGAA FRKLEYRQAI QTMQSEFFYL TTNLINDTIE LRESQTIGDQ CVYNSTHLGF QRENGTFSKY EGGVETFAHL IVLRKHGAFM LAFDLKDEKK RGLSLYAKRP DITPELREVF QKAVTHVGMD ESEIIFVDWK KDRCGQQEKK QLELGKETKK DPEEGQA. It is sometimes possible for the material contained within the vial of "Alpha-1-Acid Glycoprotein (a1AGP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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