Western Blot (WB)
(ALOX12B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).)
Rabbit anti-Human ALOX12B Polyclonal Antibody | anti-Alox12b antibody
ALOX12B, CT (ALOX12B, Arachidonate 12-lipoxygenase, 12R-type, Epidermis-type lipoxygenase 12)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(ALOX12B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).)
Western Blot (WB)
(ALOX12B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).)
Immunohistochemistry (IHC)
(ALOX12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALOX12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ALOX12B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALOX12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ALOX12B Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ALOX12B Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene can prevent the formation of the epidermal permeability barrier and cause an ichthyosiform phenotype. [provided by RefSeq, Sep 2015]
Uniprot Description
ALOX12B: Converts arachidonic acid to 12R- hydroperoxyeicosatetraenoic acid (12R-HPETE). Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE). NCIE is a non- bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume. Belongs to the lipoxygenase family.
Protein type: Lipid Metabolism - arachidonic acid; EC 1.13.11.-; Oxidoreductase
Cellular Component: cytoplasm
Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; p-cumate 2,3-dioxygenase activity; 2-aminophenol 1,6-dioxygenase activity; arachidonate 12-lipoxygenase activity; 2,3-dihydroxy-p-cumate dioxygenase activity; 3,5-dichlorocatechol 1,2-dioxygenase activity; protocatechuate 3,4-dioxygenase type II activity; hydroquinone 1,2-dioxygenase activity; metal ion binding; 5,6-dihydroxy-3-methyl-2-oxo-1,2-dihydroquinoline dioxygenase activity; oxidoreductase activity; 1,2-dihydroxyfluorene 1,1-alpha-dioxygenase activity; dihydroxyfluorene dioxygenase activity; styrene dioxygenase activity; 2,6-dichloro-p-hydroquinone 1,2-dioxygenase activity; 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase activity; catechol dioxygenase activity; 3,4-dihydroxyfluorene 4,4-alpha-dioxygenase activity; phenanthrene dioxygenase activity; 2,3-dihydroxy DDT 1,2-dioxygenase activity; 4-hydroxycatechol 1,2-dioxygenase activity; 2,2',3-trihydroxybiphenyl dioxygenase activity; chlorocatechol 1,2-dioxygenase activity; 1,2-dihydroxynaphthalene-6-sulfonate 1,8a-dioxygenase activity; 3,4-dihydroxyphenanthrene dioxygenase activity; carbazole 1,9a-dioxygenase activity; dihydroxydibenzothiophene dioxygenase activity; 2,3-dihydroxy-ethylbenzene 1,2-dioxygenase activity; dioxygenase activity; iron ion binding; 1,1-dichloro-2-(dihydroxy-4-chlorophenyl)-(4-chlorophenyl)ethene 1,2-dioxygenase activity
Biological Process: sphingolipid metabolic process; lipoxygenase pathway; positive regulation of MAPKKK cascade; protein amino acid lipidation; ceramide biosynthetic process; linoleic acid metabolic process; arachidonic acid metabolic process; lipid metabolic process; hepoxilin biosynthetic process; fatty acid metabolic process
Research Articles on Alox12b
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Product Notes
The Alox12b alox12b (Catalog #AAA6010733) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALOX12B, CT (ALOX12B, Arachidonate 12-lipoxygenase, 12R-type, Epidermis-type lipoxygenase 12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX12B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Alox12b alox12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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