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Western Blot (WB) (Western blot analysis of ALMS1 using HeLa whole cell lysates)

Rabbit anti-Human, Rat ALMS1 Polyclonal Antibody | anti-ALMS1 antibody

ALMS1 Antibody

Gene Names
ALMS1; ALSS
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ALMS1; Polyclonal Antibody; ALMS1 Antibody; Alms1; ALMS1_HUMAN; ALSS; Alstrom syndrome 1; Alstrom syndrome protein 1; anti-ALMS1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ALMS1 antibody detects endogenous levels of total ALMS1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
4169
Applicable Applications for anti-ALMS1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
Immunogen
A synthesized peptide derived from human ALMS1
Subcellular Location
Cytoplasm. Cytoplasm > Cytoskeleton > Centrosome. Cytoplasm > Cytoskeleton > Cilium Basal Body. Cytoplasm > Cytoskeleton > Spindle Pole. Associated with centrosomes and basal body at the base of primary cilia. During mitosis localizes to both spindle poles.
Tissue Specificity
Expressed in all tissues tested including adipose and pancreas. Expressed by beta-cells of the islets in the pancreas (at protein level).
Predicted Cross Reactivity
Xenopus
Similarity
Xenopus (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ALMS1 using HeLa whole cell lysates)

Western Blot (WB) (Western blot analysis of ALMS1 using HeLa whole cell lysates)

Immunohistochemistry (IHC)

(MBS9609514 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9609514 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ALMS1 antibody
Function: Involved in PCM1-dependent intracellular transport. Required, directly or indirectly, for the localization of NCAPD2 to the proximal ends of centrioles. Required for proper formation and/or maintenance of primary cilia (PC), microtubule-based structures that protrude from the surface of epithelial cells.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 461 kDa
Predicted: 462 kDa
NCBI Official Full Name
Alstrom syndrome protein 1
NCBI Official Synonym Full Names
ALMS1, centrosome and basal body associated protein
NCBI Official Symbol
ALMS1
NCBI Official Synonym Symbols
ALSS
NCBI Protein Information
Alstrom syndrome protein 1
UniProt Protein Name
Alstrom syndrome protein 1
Protein Family
UniProt Gene Name
ALMS1
UniProt Synonym Gene Names
KIAA0328

NCBI Description

This gene encodes a protein containing a large tandem-repeat domain as well as additional low complexity regions. The encoded protein functions in microtubule organization, particularly in the formation and maintanance of cilia. Mutations in this gene cause Alstrom syndrome. There is a pseudogene for this gene located adjacent in the same region of chromosome 2. Alternative splice variants have been described but their full length nature has not been determined. [provided by RefSeq, Apr 2014]

Uniprot Description

Involved in PCM1-dependent intracellular transport. Required, directly or indirectly, for the localization of NCAPD2 to the proximal ends of centrioles. Required for proper formation and/or maintenance of primary cilia (PC), microtubule-based structures that protrude from the surface of epithelial cells.

Research Articles on ALMS1

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Product Notes

The ALMS1 alms1 (Catalog #AAA9609514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALMS1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ALMS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the ALMS1 alms1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALMS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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